Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Mycolicibacterium phlei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | enzyme extremely sensitive, NADH and Ca2+ protect | Mycolicibacterium phlei | |
p-chloromercuribenzoate | enzyme extremely sensitive, NADH and Ca2+ protect | Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
NAD+ | - |
Mycolicibacterium phlei | |
7.4 | - |
(R)-3-hydroxybutanoate | - |
Mycolicibacterium phlei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Mycolicibacterium phlei | - |
acetoacetate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose | Mycolicibacterium phlei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Mycolicibacterium phlei | acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Mycolicibacterium phlei | acetoacetate + NADH + H+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
loss of 70% activity after 15 min, 0.24 mg protein/ml, Ca2+ and Mn2+ stabilize enzyme | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7 | reduction of acetoacetate | Mycolicibacterium phlei |
8.4 | - |
oxidation of beta-hydroxybutyrate | Mycolicibacterium phlei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | oxidation of beta-hydroxybutyrate | Mycolicibacterium phlei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycolicibacterium phlei |