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Literature summary for 1.1.1.299 extracted from

  • Roche, J.; Girard, E.; Mas, C.; Madern, D.
    The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization (2019), J. Struct. Biol., 208, 7-17 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE) cells Ignicoccus islandicus

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, sitting drop vapor diffusion method, using 10% (w/v) PEG 6000 and 100 mM EPES at pH 7.0 Ignicoccus islandicus

Inhibitors

Inhibitors Comment Organism Structure
oxaloacetate
-
Ignicoccus islandicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
oxaloacetate at pH 7.0 and 70°C Ignicoccus islandicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
124000
-
size exclusion chromatography-multi angle laser light scattering Ignicoccus islandicus
133000
-
calculated from amino acid sequence Ignicoccus islandicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADH + H+ Ignicoccus islandicus
-
(S)-malate + NAD+
-
?
oxaloacetate + NADPH + H+ Ignicoccus islandicus
-
(S)-malate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Ignicoccus islandicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and Superpose 12 gel filtration Ignicoccus islandicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Ignicoccus islandicus (S)-malate + NAD+
-
?
oxaloacetate + NADH + H+ the enzyme strongly prefers oxaloacetate by three orders of magnitude compared to pyruvate Ignicoccus islandicus (S)-malate + NAD+
-
?
oxaloacetate + NADPH + H+
-
Ignicoccus islandicus (S)-malate + NADP+
-
?
pyruvate + NADH + H+ weak catalytic efficiency with pyruvate Ignicoccus islandicus ?
-
?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Ignicoccus islandicus

Synonyms

Synonyms Comment Organism
LDH-like MalDH
-
Ignicoccus islandicus
NAD(P)-dependent malate dehydrogenase
-
Ignicoccus islandicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
pyruvate at pH 6.0 and 70°C Ignicoccus islandicus
444
-
oxaloacetate at pH 7.0 and 70°C Ignicoccus islandicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ignicoccus islandicus
NADP+
-
Ignicoccus islandicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
oxaloacetate at pH 7.0 and 70°C Ignicoccus islandicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
oxaloacetate at pH 7.0 and 70°C Ignicoccus islandicus