BRENDA - Enzyme Database
show all sequences of 1.1.1.299

Two malate dehydrogenases in Methanobacterium thermoautotrophicum

Thompson, H.; Tersteegen, A.; Thauer, R.; Hedderich, R.; Arch. Microbiol. 170, 38-42 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NADH
inhibitory above 0.2 mM
Methanothermobacter thermautotrophicus
NADPH
inhibitory above 0.2 mM
Methanothermobacter thermautotrophicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADPH
pH 7.6
Methanothermobacter thermautotrophicus
0.09
-
NADH
pH 7.6
Methanothermobacter thermautotrophicus
0.09
-
oxaloacetate
pH 7.6
Methanothermobacter thermautotrophicus
0.4
-
malate
pH 7.6
Methanothermobacter thermautotrophicus
0.4
-
malate
at pH 7.6 and 60°C
Methanothermobacter thermautotrophicus
0.5
-
NAD+
pH 7.6
Methanothermobacter thermautotrophicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
36700
-
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
Organism
Organism
UniProt
Commentary
Textmining
Methanothermobacter thermautotrophicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methanothermobacter thermautotrophicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1
-
pH 7.6, oxidation of malate, cosubstrate NAD+
Methanothermobacter thermautotrophicus
1
-
pH 7.6, oxidation of malate, cosubstrate NADP+
Methanothermobacter thermautotrophicus
52
-
pH 7.6, reduction of oxaloacetate, cosubstrate NADP+
Methanothermobacter thermautotrophicus
60
-
pH 7.6, reduction of oxaloacetate, cosubstrate NAD+
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-malate + NAD+
-
695913
Methanothermobacter thermautotrophicus
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NADP+
-
695913
Methanothermobacter thermautotrophicus
oxaloacetate + NADPH + H+
-
-
-
?
additional information
no substrate: pyruvate
695913
Methanothermobacter thermautotrophicus
?
-
-
-
?
oxaloacetate + NADH
-
695913
Methanothermobacter thermautotrophicus
L-malate + NAD+
-
-
-
?
oxaloacetate + NADPH
-
695913
Methanothermobacter thermautotrophicus
L-malate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
Synonyms
Synonyms
Commentary
Organism
MDH II
uses both NADP+ and NAD+
Methanothermobacter thermautotrophicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Methanothermobacter thermautotrophicus
NADH
-
Methanothermobacter thermautotrophicus
NADP+
-
Methanothermobacter thermautotrophicus
NADPH
-
Methanothermobacter thermautotrophicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Methanothermobacter thermautotrophicus
NADH
-
Methanothermobacter thermautotrophicus
NADP+
-
Methanothermobacter thermautotrophicus
NADPH
-
Methanothermobacter thermautotrophicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADH
inhibitory above 0.2 mM
Methanothermobacter thermautotrophicus
NADPH
inhibitory above 0.2 mM
Methanothermobacter thermautotrophicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADPH
pH 7.6
Methanothermobacter thermautotrophicus
0.09
-
NADH
pH 7.6
Methanothermobacter thermautotrophicus
0.09
-
oxaloacetate
pH 7.6
Methanothermobacter thermautotrophicus
0.4
-
malate
pH 7.6
Methanothermobacter thermautotrophicus
0.4
-
malate
at pH 7.6 and 60°C
Methanothermobacter thermautotrophicus
0.5
-
NAD+
pH 7.6
Methanothermobacter thermautotrophicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
36700
-
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanothermobacter thermautotrophicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1
-
pH 7.6, oxidation of malate, cosubstrate NAD+
Methanothermobacter thermautotrophicus
1
-
pH 7.6, oxidation of malate, cosubstrate NADP+
Methanothermobacter thermautotrophicus
52
-
pH 7.6, reduction of oxaloacetate, cosubstrate NADP+
Methanothermobacter thermautotrophicus
60
-
pH 7.6, reduction of oxaloacetate, cosubstrate NAD+
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-malate + NAD+
-
695913
Methanothermobacter thermautotrophicus
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NADP+
-
695913
Methanothermobacter thermautotrophicus
oxaloacetate + NADPH + H+
-
-
-
?
additional information
no substrate: pyruvate
695913
Methanothermobacter thermautotrophicus
?
-
-
-
?
oxaloacetate + NADH
-
695913
Methanothermobacter thermautotrophicus
L-malate + NAD+
-
-
-
?
oxaloacetate + NADPH
-
695913
Methanothermobacter thermautotrophicus
L-malate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE, x * 36700, calculated
Methanothermobacter thermautotrophicus
Other publictions for EC 1.1.1.299
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668012
Oikawa
Purification, characterization ...
Flavobacterium frigidimaris, Flavobacterium frigidimaris KUC-1
Biosci. Biotechnol. Biochem.
69
2146-2154
2005
-
-
-
-
-
-
5
8
-
-
2
-
-
2
-
-
-
1
-
-
1
-
7
1
-
1
1
2
8
2
2
1
4
-
-
-
-
-
-
4
-
-
-
-
5
-
8
-
-
2
-
-
-
-
-
-
-
1
-
7
1
1
1
2
8
2
2
1
-
-
-
-
-
2
2
695913
Thompson
Two malate dehydrogenases in M ...
Methanothermobacter thermautotrophicus
Arch. Microbiol.
170
38-42
1998
-
-
-
-
-
-
2
6
-
-
2
-
-
1
-
-
1
-
-
-
4
-
5
1
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
2
-
6
-
-
2
-
-
-
-
1
-
-
4
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-