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show all sequences of 1.1.1.299

Purification, characterization, and overexpression of psychrophilic and thermolabile malate dehydrogenase of a novel antarctic psychrotolerant, Flavobacterium frigidimaris KUC-1

Oikawa, T.; Yamamoto, N.; Shimoke, K.; Uesato, S.; Ikeuchi, T.; Fujioka, T.; Biosci. Biotechnol. Biochem. 69, 2146-2154 (2005)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuCl2
1 mM, 10% residual activity
Flavobacterium frigidimaris
HgCl2
1 mM, no residual activity
Flavobacterium frigidimaris
iodoacetate
1 mM, 16.4% residual activity
Flavobacterium frigidimaris
N-ethylmaleimide
1 mM, no residual activity
Flavobacterium frigidimaris
ZnCl2
1 mM, 27% residual activity
Flavobacterium frigidimaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
NAD+
10°C
Flavobacterium frigidimaris
0.029
-
NAD+
20°C
Flavobacterium frigidimaris
0.03
-
NAD+
30°C
Flavobacterium frigidimaris
0.038
-
NAD+
40°C
Flavobacterium frigidimaris
0.269
-
L-malate
20°C
Flavobacterium frigidimaris
0.288
-
L-malate
30°C
Flavobacterium frigidimaris
0.543
-
L-malate
10°C
Flavobacterium frigidimaris
0.74
-
L-malate
40°C
Flavobacterium frigidimaris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Flavobacterium frigidimaris
123000
-
gel filtration
Flavobacterium frigidimaris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium frigidimaris
-
-
-
Flavobacterium frigidimaris KUC-1
-
-
-
Reaction
Reaction
Commentary
Organism
(S)-malate + NAD(P)+ = oxaloacetate + NAD(P)H + H+
ordered bi-bi mechanism
Flavobacterium frigidimaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
176
-
30°C
Flavobacterium frigidimaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
668012
Flavobacterium frigidimaris
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NAD+
-
668012
Flavobacterium frigidimaris KUC-1
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NADP+
-
668012
Flavobacterium frigidimaris
oxaloacetate + NADPH + H+
-
-
-
?
(S)-malate + NADP+
-
668012
Flavobacterium frigidimaris KUC-1
oxaloacetate + NADPH + H+
-
-
-
?
additional information
enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate
668012
Flavobacterium frigidimaris
?
-
-
-
-
additional information
enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate
668012
Flavobacterium frigidimaris KUC-1
?
-
-
-
-
oxaloacetate + NADH + H+
-
668012
Flavobacterium frigidimaris
L-malate + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 32000, SDS-PAGE
Flavobacterium frigidimaris
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
highest initial velocity
Flavobacterium frigidimaris
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
60
-
Flavobacterium frigidimaris
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
half-life 26 min
Flavobacterium frigidimaris
40
-
half-life 3 min
Flavobacterium frigidimaris
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
41
-
NAD+
10°C
Flavobacterium frigidimaris
41.3
-
L-malate
10°C
Flavobacterium frigidimaris
48.4
-
L-malate
20°C
Flavobacterium frigidimaris
48.6
-
NAD+
20°C
Flavobacterium frigidimaris
83.2
-
L-malate
30°C
Flavobacterium frigidimaris
83.4
-
NAD+
30°C
Flavobacterium frigidimaris
138
-
L-malate
40°C
Flavobacterium frigidimaris
139
-
NAD+
40°C
Flavobacterium frigidimaris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction of oxaloacetate
Flavobacterium frigidimaris
10.5
-
oxidation of malate
Flavobacterium frigidimaris
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
10
reduction of oxaloacetate
Flavobacterium frigidimaris
6.5
12
oxidation of malate
Flavobacterium frigidimaris
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
10.5
-
Flavobacterium frigidimaris
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADPH
Flavobacterium frigidimaris
NAD+
relative activity of NAD+ to NADP+ is 43.9%
Flavobacterium frigidimaris
NADH
-
Flavobacterium frigidimaris
NADP+
relative activity of NAD+ to NADP+ is 43.9%
Flavobacterium frigidimaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADPH
Flavobacterium frigidimaris
NAD+
relative activity of NAD+ to NADP+ is 43.9%
Flavobacterium frigidimaris
NADH
-
Flavobacterium frigidimaris
NADP+
relative activity of NAD+ to NADP+ is 43.9%
Flavobacterium frigidimaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuCl2
1 mM, 10% residual activity
Flavobacterium frigidimaris
HgCl2
1 mM, no residual activity
Flavobacterium frigidimaris
iodoacetate
1 mM, 16.4% residual activity
Flavobacterium frigidimaris
N-ethylmaleimide
1 mM, no residual activity
Flavobacterium frigidimaris
ZnCl2
1 mM, 27% residual activity
Flavobacterium frigidimaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.028
-
NAD+
10°C
Flavobacterium frigidimaris
0.029
-
NAD+
20°C
Flavobacterium frigidimaris
0.03
-
NAD+
30°C
Flavobacterium frigidimaris
0.038
-
NAD+
40°C
Flavobacterium frigidimaris
0.269
-
L-malate
20°C
Flavobacterium frigidimaris
0.288
-
L-malate
30°C
Flavobacterium frigidimaris
0.543
-
L-malate
10°C
Flavobacterium frigidimaris
0.74
-
L-malate
40°C
Flavobacterium frigidimaris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Flavobacterium frigidimaris
123000
-
gel filtration
Flavobacterium frigidimaris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
176
-
30°C
Flavobacterium frigidimaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
668012
Flavobacterium frigidimaris
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NAD+
-
668012
Flavobacterium frigidimaris KUC-1
oxaloacetate + NADH + H+
-
-
-
?
(S)-malate + NADP+
-
668012
Flavobacterium frigidimaris
oxaloacetate + NADPH + H+
-
-
-
?
(S)-malate + NADP+
-
668012
Flavobacterium frigidimaris KUC-1
oxaloacetate + NADPH + H+
-
-
-
?
additional information
enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate
668012
Flavobacterium frigidimaris
?
-
-
-
-
additional information
enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate
668012
Flavobacterium frigidimaris KUC-1
?
-
-
-
-
oxaloacetate + NADH + H+
-
668012
Flavobacterium frigidimaris
L-malate + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 32000, SDS-PAGE
Flavobacterium frigidimaris
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
highest initial velocity
Flavobacterium frigidimaris
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
60
-
Flavobacterium frigidimaris
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
half-life 26 min
Flavobacterium frigidimaris
40
-
half-life 3 min
Flavobacterium frigidimaris
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
41
-
NAD+
10°C
Flavobacterium frigidimaris
41.3
-
L-malate
10°C
Flavobacterium frigidimaris
48.4
-
L-malate
20°C
Flavobacterium frigidimaris
48.6
-
NAD+
20°C
Flavobacterium frigidimaris
83.2
-
L-malate
30°C
Flavobacterium frigidimaris
83.4
-
NAD+
30°C
Flavobacterium frigidimaris
138
-
L-malate
40°C
Flavobacterium frigidimaris
139
-
NAD+
40°C
Flavobacterium frigidimaris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction of oxaloacetate
Flavobacterium frigidimaris
10.5
-
oxidation of malate
Flavobacterium frigidimaris
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
10
reduction of oxaloacetate
Flavobacterium frigidimaris
6.5
12
oxidation of malate
Flavobacterium frigidimaris
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
10.5
-
Flavobacterium frigidimaris
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
289
-
L-malate
30°C
Flavobacterium frigidimaris
2790
-
NAD+
30°C
Flavobacterium frigidimaris
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
289
-
L-malate
30°C
Flavobacterium frigidimaris
2790
-
NAD+
30°C
Flavobacterium frigidimaris
Other publictions for EC 1.1.1.299
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668012
Oikawa
Purification, characterization ...
Flavobacterium frigidimaris, Flavobacterium frigidimaris KUC-1
Biosci. Biotechnol. Biochem.
69
2146-2154
2005
-
-
-
-
-
-
5
8
-
-
2
-
-
2
-
-
-
1
-
-
1
-
7
1
1
1
2
8
2
2
1
4
-
-
-
-
-
-
4
-
-
-
-
5
-
8
-
-
2
-
-
-
-
-
-
-
1
-
7
1
1
1
2
8
2
2
1
-
-
-
-
-
2
2
695913
Thompson
Two malate dehydrogenases in M ...
Methanothermobacter thermautotrophicus
Arch. Microbiol.
170
38-42
1998
-
-
-
-
-
-
2
5
-
-
2
-
-
1
-
-
1
-
-
-
3
-
5
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
2
-
5
-
-
2
-
-
-
-
1
-
-
3
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-