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Literature summary for 1.1.1.298 extracted from

  • Takayama, S.; Ozaki, A.; Konishi, R.; Otomo, C.; Kishida, M.; Hirata, Y.; Matsumoto, T.; Tanaka, T.; Kondo, A.
    Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe (2018), Microb. Cell Fact., 17, 176 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene mcr, separate recombinant expression of the N- and C-terminal subdomains of the enzyme, MCR-C and MCR-N, in Schizosaccharomyces pombe strain FY12804/NBRP, coexpression of acetyl-CoA synthase, gene acsSE, and aldehyde dehydrogenase, gene atd1, under the control of the cam1 promoter Chloroflexus aurantiacus

Protein Variants

Protein Variants Comment Organism
additional information enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. 3-HP production from glucose and cellobiose via the malonyl-CoA pathway, the mcr gene, encoding the bifunctional malonyl-CoA reductase of Chloroflexus aurantiacus, is dissected into two functionally distinct fragments, and the activities of the encoded protein are balanced. The MCR-C fragment reduces malonyl-CoA to malonate semialdehyde, while the MCR-N fragment reduces malonate semialdehyde to 3-HP. To increase the cellular supply of malonyl-CoA and acetyl-CoA, genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase are introduced. The resulting strain produces 3-HP at 1.0 g/l from a culture starting at a glucose concentration of 50 g/l. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50 g/l cellobiose, the beta-glucosidase-displaying strain consumes cellobiose efficiently and produces 3-HP at 3.5 g/l. Under fed-batch conditions starting from cellobiose, this strain produces 3-HP at up to 11.4 g/l, corresponding to a yield of 11.2% Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonate semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
 3-hydroxypropanoate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus Q6QQP7 bifunctional enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate semialdehyde + NADPH + H+
-
 Chloroflexus aurantiacus 3-hydroxypropanoate + NADP+
-
 ?
additional information the bifunctional enzyme shows malonate semialdehyde reduction activity and also malonyl-CoA reduction activity, EC 1.2.1.75. The C-terminal subdomain MCR-C reduces malonyl-CoA to malonate semialdehyde, while the N-terminal subdomain MCR-N reduces malonate semialdehyde to 3-HP Chloroflexus aurantiacus ?
-
-

Synonyms

Synonyms Comment Organism
malonyl-CoA reductase UniProt Chloroflexus aurantiacus
MCR
-
 Chloroflexus aurantiacus
More see also EC 1.2.1.75 Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Chloroflexus aurantiacus