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Literature summary for 1.1.1.294 extracted from

  • Xu, B.; Li, H.; Li, Y.; Yu, G.; Zhang, J.; Huang, B.
    Characterization and transcriptional regulation of chlorophyll b reductase gene non-yellow coloring 1 associated with leaf senescence in perennial ryegrass (Lolium perenne L.) (2018), Environ. Exp. Bot., 149, 43-50 .
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Cloned(Commentary)

Cloned (Comment) Organism
gene NYC1, cloned using the RACE-PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semiquantitative and quantitative RT-PCR enzyme expression analysis, transient overexpression of LpNYC1 in wild-type Nicotiana benthamiana under control of CaMV 35S promoter and in Arabidopsis thaliana nyc1 null mutant strain AGL1, both via Agrobacterium tumefaciens transfection, recombinant expression of GFP-tagged enzyme NYC1 in Lolium perenne protoplasts Lolium perenne

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Lolium perenne 9507
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thylakoid membrane
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 Lolium perenne 42651
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide b + NAD(P)H + H+ Lolium perenne
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 71-hydroxychlorophyllide a + NAD(P)+
-
 r

Organism

Organism UniProt Comment Textmining
Lolium perenne A0A1Q1NIA8 cv. Pinnacle
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Source Tissue

Source Tissue Comment Organism Textmining
leaf expression in leaf sheaths is detectable but is significantly lower than those in expanding and mature leaves. During the progression of natural leaf senescence at 24, 30, and 36 d after leaf emergence, the expression levels of LpNYC1 increase and are approximately 23.1, 38.8, and 148.7times higher than those at the expanding stage (12 d after leaf emergence), respectively Lolium perenne
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additional information the expression pattern is correlated with the progression of leaf senescence Lolium perenne
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root low expression level Lolium perenne
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stem low expression level Lolium perenne
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide b + NAD(P)H + H+
-
 Lolium perenne 71-hydroxychlorophyllide a + NAD(P)+
-
 r

Synonyms

Synonyms Comment Organism
chlorophyll b reductase
-
 Lolium perenne
LpNYC1
-
 Lolium perenne
NON-YELLOW COLORING 1
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 Lolium perenne
NYC1
-
 Lolium perenne

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme sequence harbors the TGXXGXXG cofactor binding motif Lolium perenne
NAD(P)+
-
 Lolium perenne
NAD(P)H
-
 Lolium perenne

Expression

Organism Comment Expression
Lolium perenne expression of LpNYC1 is suppressed by treatment with AVG (ethylene biosynthesis inhibitor) down
Lolium perenne expression of LpNYC1 is highly inducible by abscisic acid (ABA) and ethephon (ethylene releasing reagent). Transcription factors LpABI5, LpABF3, and LpEIN3 directly activate the expression of LpNYC1 by binding to its promoter up

General Information

General Information Comment Organism
evolution LpNYC1 shares the highest amino acid sequence similarity with BdNYC1 (91.5%) in Brachypodium distachyon, and the lowest with Arabidopsis NYC1 (63.6%). Despite the sequence divergence, the NYC1 orthologues all share the classical chloroplast-localized short-chain dehydrogenase/reductase (SDR) domain with the TGXXGXXG cofactor binding motif and the YXXXK active site for catabolizing Chl b into 7-hydroxymethyl-chl a Lolium perenne
malfunction knocking out the Chl b reductase gene might lead to a stay-green phenotype. Overexpression of LpNYC1 activates leaf senescence in Nicotiana benthamiana and rescues the stay-green trait in the Arabidopsis thaliana nyc1 null mutant Lolium perenne
physiological function chlorophyll (Chl) degradation leads to leaf senescence and adversely affects biomass production of forage grasses and aesthetic appearance of turfgrasses. Transcriptional regulation of chlorophyll b reductase gene non-yellow coloring 1 associated with leaf senescence in perennial ryegrass. Predicted functional roles of LpNYC1 in Chl catabolism in green tissues and in leaf senescence. LpNYC1 catalyzes Chl degradation. Upstream transcription factors from Lolium perenne, LpABI5, LpABF3, and LpEIN3, directly regulating the expression of LpNYC1 in perennial ryegrass during leaf senescence Lolium perenne