BRENDA - Enzyme Database
show all sequences of 1.1.1.291

Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri

Alhapel, A.; Darley, D.J.; Wagener, N.; Eckel, E.; Elsner, N.; Pierik, A.J.; Proc. Natl. Acad. Sci. USA 103, 12341-12346 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Eubacterium barkeri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NAD+
-
Eubacterium barkeri
1.1
-
2-hydroxymethylglutarate
-
Eubacterium barkeri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Eubacterium barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-formylglutarate + NADH + H+
Eubacterium barkeri
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
2-hydroxymethylglutarate + NAD+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Eubacterium barkeri
-
-
-
Oxidation Stability
Oxidation Stability
Organism
instability under anaerobic conditions
Eubacterium barkeri
Purification (Commentary)
Purification (Commentary)
Organism
-
Eubacterium barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-formylglutarate + NADH + H+
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
670692
Eubacterium barkeri
2-hydroxymethylglutarate + NAD+
-
-
-
?
2-hydroxymethylglutarate + NAD+
-
670692
Eubacterium barkeri
2-formylglutarate + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 32000, SDS-PAGE
Eubacterium barkeri
Synonyms
Synonyms
Commentary
Organism
HgD
-
Eubacterium barkeri
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Eubacterium barkeri
NADH
-
Eubacterium barkeri
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Eubacterium barkeri
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Eubacterium barkeri
NADH
-
Eubacterium barkeri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NAD+
-
Eubacterium barkeri
1.1
-
2-hydroxymethylglutarate
-
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Eubacterium barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-formylglutarate + NADH + H+
Eubacterium barkeri
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
2-hydroxymethylglutarate + NAD+
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
instability under anaerobic conditions
Eubacterium barkeri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-formylglutarate + NADH + H+
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
670692
Eubacterium barkeri
2-hydroxymethylglutarate + NAD+
-
-
-
?
2-hydroxymethylglutarate + NAD+
-
670692
Eubacterium barkeri
2-formylglutarate + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 32000, SDS-PAGE
Eubacterium barkeri
Other publictions for EC 1.1.1.291
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670692
Alhapel
Molecular and functional analy ...
Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
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