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Literature summary for 1.1.1.29 extracted from

  • Liu, Y.; Guerard, F.; Hodges, M.; Jossier, M.
    Phosphomimetic T335D mutation of hydroxypyruvate reductase 1 modifies cofactor specificity and impacts Arabidopsis growth in air (2020), Plant Physiol., 183, 194-205 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T335A nonphosphorylatable variant Arabidopsis thaliana
T335D phosphomimetic variant, exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
0.018
-
NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
0.028
-
NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
0.102
-
NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
0.135
-
NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
0.147
-
NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
0.167
-
Hydroxypyruvate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
0.173
-
Hydroxypyruvate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
0.22
-
Hydroxypyruvate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
0.995
-
Hydroxypyruvate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.428
-
Hydroxypyruvate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.579
-
Hydroxypyruvate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Arabidopsis thaliana 5777
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C9W5 catalyzes reactions of EC 1.1.1.29 and EC 1.1.1.26
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NADH + H+ reaction of EC 1.1.1.26 Arabidopsis thaliana glycolate + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Arabidopsis thaliana D-glycerate + NAD+
-
?
hydroxypyruvate + NADPH + H+ NADPH is a poor cofactor for wild-type Arabidopsis thaliana D-glycerate + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
139
-
Hydroxypyruvate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
144
-
NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
148
-
NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
150
-
Hydroxypyruvate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
160
-
Hydroxypyruvate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
162
-
NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
206
-
Hydroxypyruvate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
218
-
NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
230
-
NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
248
-
NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
253
-
Hydroxypyruvate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
284
-
Hydroxypyruvate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
Arabidopsis thaliana
NADPH NADPH is a poor cofactor for wild-type Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism HPR1 phosphomimetic variant T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity but improved NADPH-dependent activity. Complementation of the Arabidopsis HPR1 mutant by either wild-type HPR1 or HPR1 nonphosphorylatable mutant T335A fully complements the photorespiratory growth phenotype of the HPR1 mutant in ambient air, whereas HPR1 T335D-containing HPR1 mutant plants remain smaller and have lower photosynthetic CO2 assimilation rates. These phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1 T335D-complemented HPR1 mutant rosettes compared to all other HPR1-containing lines Arabidopsis thaliana