Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BIVU0811 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | about 72% residual activity at 2 mM | Arabidopsis thaliana | |
additional information | not inhibited by H2O2 | Arabidopsis thaliana | |
S-nitrosoglutathione | about 80% residual activity at 2 mM | Arabidopsis thaliana | |
SIN-1 | about 35% residual activity at 1 mM | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42300 | - |
- |
Arabidopsis thaliana |
63500 | - |
- |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADH + H+ | Arabidopsis thaliana | - |
D-glycerate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C9W5 | - |
- |
Purification (Comment) | Organism |
---|---|
LYTRA column chromatography, and Superdex 200 gel filtration | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0051 | - |
recombinant enzyme, at pH and °C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADH + H+ | - |
Arabidopsis thaliana | D-glycerate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42300, calculated from amino acid sequence | Arabidopsis thaliana |
? | x * 63500, enzyme with N-terminal choline binding domain, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
HPR1 | - |
Arabidopsis thaliana |
hydroxypyruvate reductase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana |