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Literature summary for 1.1.1.289 extracted from

  • Soemphol, W.; Toyama, H.; Moonmangmee, D.; Adachi, O.; Matsushita, K.
    L-sorbose reductase and its transcriptional regulator involved in L-sorbose utilization of Gluconobacter frateurii (2007), J. Bacteriol., 189, 4800-4808.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes sldSLC and sboA, DNA and amino acid sequence determination and analysis, genetic organization, a putational transcription regulator encoded by gene sboR is located upstream, sboRA comprises an operon, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Gluconobacter frateurii

Protein Variants

Protein Variants Comment Organism
additional information construction of disruption mutants, a mutant defective in sboA shows significantly reduced growth on L-sorbose, while a sboR mutant grows on L-sorbose even better than the wild-type strain with higher NADPH-SR activity in cytoplasm fractions Gluconobacter frateurii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Gluconobacter frateurii 5737
-
membrane
-
Gluconobacter frateurii 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28320
-
3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii
29000
-
3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii
87000
-
recombinant SboA, gel filtration Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucitol + NADP+ Gluconobacter frateurii
-
D-sorbose + NADPH + H+
-
r
D-glucitol + NADP+ Gluconobacter frateurii
-
L-sorbose + NADPH + H+
-
r
D-glucitol + NADP+ Gluconobacter frateurii THD32
-
D-sorbose + NADPH + H+
-
r
D-glucitol + NADP+ Gluconobacter frateurii THD32
-
L-sorbose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Gluconobacter frateurii
-
genes sldSLC and sboA
-
Gluconobacter frateurii THD32
-
genes sldSLC and sboA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information SboA is required for effective growth on D- or L-sorbose, the organism also grows on glycerol Gluconobacter frateurii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucitol + NADP+
-
Gluconobacter frateurii D-sorbose + NADPH + H+
-
r
D-glucitol + NADP+
-
Gluconobacter frateurii THD32 D-sorbose + NADPH + H+
-
r
D-glucitol + NADP+
-
Gluconobacter frateurii L-sorbose + NADPH + H+
-
r
D-glucitol + NADP+
-
Gluconobacter frateurii THD32 L-sorbose + NADPH + H+
-
r

Subunits

Subunits Comment Organism
trimer 3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii

Synonyms

Synonyms Comment Organism
FAD-dependent D-sorbitol dehydrogenase
-
Gluconobacter frateurii
flavin adenine dinucleotide-dependent D-sorbitol dehydrogenase
-
Gluconobacter frateurii
NADPH-dependent L-sorbose reductase
-
Gluconobacter frateurii
NADPH-SR
-
Gluconobacter frateurii
SboA
-
Gluconobacter frateurii
SLDH
-
Gluconobacter frateurii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 oxidation of L-sorbitol Gluconobacter frateurii
9
-
reduction reaction, assay at Gluconobacter frateurii
10
-
oxidation of D-sorbitol Gluconobacter frateurii

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Gluconobacter frateurii
NADP+ dependent on Gluconobacter frateurii
NADPH dependent on Gluconobacter frateurii

pI Value

Organism Comment pI Value Maximum pI Value
Gluconobacter frateurii SboA, sequence calculation
-
5.72