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Literature summary for 1.1.1.287 extracted from
- Molecular cloning and functional expression of D-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli (2005), FEMS Microbiol. Lett., 252, 35-42.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ArDH, DNA and amino acid sequence determination and analysis, gene ArDH is part of an operon with several components of a sugar transporter system, subcloning in Escherichia coli strain TG-1, functional expression in Escherichia coli strain BL21(DE3) as His6-tagged enzyme | Gluconobacter oxydans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
D-arabitol | pH 8.5, 25°C | Gluconobacter oxydans |  |
| 18.5 | - |
D-mannitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 25 | - |
D-xylulose | pH 6.0, 25°C | Gluconobacter oxydans | |
| 47 | - |
D-fructose | pH 6.0, 25°C | Gluconobacter oxydans | |
| 125 | - |
D-sorbitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 266 | - |
meso-erythritol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 384 | - |
glycerol | pH 8.5, 25°C | Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Gluconobacter oxydans | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, recombinant enzyme, SDS-PAGE | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabitinol + NADP+ | Gluconobacter oxydans | - |
D-xylulose + NADPH + H+ | - |
? | |
| D-arabitinol + NADP+ | Gluconobacter oxydans CGMCC 1.110 | - |
D-xylulose + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | Q58LW6 | - |
- |
| Gluconobacter oxydans CGMCC 1.110 | Q58LW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli | Gluconobacter oxydans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:arabitol-grown cell | - |
Gluconobacter oxydans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
purified recombinant enzyme, substrate glycerol | Gluconobacter oxydans |
| 3.2 | - |
purified recombinant enzyme, substrate meso-erythritol | Gluconobacter oxydans |
| 6.4 | - |
purified recombinant enzyme, substrate D-sorbitol | Gluconobacter oxydans |
| 7.9 | - |
purified recombinant enzyme, substrate D-fructose | Gluconobacter oxydans |
| 11.4 | - |
purified recombinant enzyme, substrate D-xylulose | Gluconobacter oxydans |
| 23.5 | - |
purified recombinant enzyme, substrate D-mannitol | Gluconobacter oxydans |
| 27.6 | - |
purified recombinant enzyme, substrate D-arabitol | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabitinol + NADP+ | - |
Gluconobacter oxydans | D-xylulose + NADPH + H+ | - |
? | |
| D-arabitinol + NADP+ | - |
Gluconobacter oxydans CGMCC 1.110 | D-xylulose + NADPH + H+ | - |
? | |
| D-arabitol + NADP+ | preferred substrate for the forward and reverse reaction, respectively | Gluconobacter oxydans | D-xylulose + NADPH + H+ | - |
r | |
| D-arabitol + NADP+ | preferred substrate for the forward and reverse reaction, respectively | Gluconobacter oxydans CGMCC 1.110 | D-xylulose + NADPH + H+ | - |
r | |
| D-mannitol + NADP+ | preferred substrate for the forward and reverse reaction, respectively | Gluconobacter oxydans | D-fructose + NADPH + H+ | - |
r | |
| D-mannitol + NADP+ | preferred substrate for the forward and reverse reaction, respectively | Gluconobacter oxydans CGMCC 1.110 | D-fructose + NADPH + H+ | - |
r | |
| D-sorbitol + NADP+ | - |
Gluconobacter oxydans | sorbose + NADPH + H+ | - |
r | |
| D-sorbitol + NADP+ | - |
Gluconobacter oxydans CGMCC 1.110 | sorbose + NADPH + H+ | - |
r | |
| glycerol + NADP+ | - |
Gluconobacter oxydans | ? + NADPH + H+ | - |
r | |
| meso-erythritol + NADP+ | - |
Gluconobacter oxydans | L-erythrose + NADPH + H+ | - |
r | |
| additional information | substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol | Gluconobacter oxydans | ? | - |
? | |
| additional information | substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol | Gluconobacter oxydans CGMCC 1.110 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, recombinant enzyme, SDS-PAGE | Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArDH | - |
Gluconobacter oxydans |
| D-arabitol dehydrogenase | - |
Gluconobacter oxydans |
| More | the enzyme belongs to the short-chain dehydrogenase family | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gluconobacter oxydans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.28 | - |
meso-erythritol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 1.33 | - |
glycerol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 2.57 | - |
D-sorbitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 196 | - |
D-xylulose | pH 6.0, 25°C | Gluconobacter oxydans | |
| 224 | - |
D-fructose | pH 6.0, 25°C | Gluconobacter oxydans | |
| 243 | - |
D-mannitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 258 | - |
D-arabitol | pH 8.5, 25°C | Gluconobacter oxydans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
reverse reaction | Gluconobacter oxydans |
| 8.5 | - |
forward reaction | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | dependent on | Gluconobacter oxydans | |
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