Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
R85V | complete loss of activity with citrate, retains activity with isocitrate | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.405 | - |
isocitrate | 60°C, pH 8.0 | Thermus thermophilus | |
7.486 | - |
homoisocitrate | 60°C, pH 8.0 | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72IW9 | expression in Escherichia coli | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72IW9 | expression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homoisocitrate + NAD+ | - |
Thermus thermophilus | ? + NADH | - |
? | |
homoisocitrate + NAD+ | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? + NADH | - |
? | |
isocitrate + NAD+ | about 20times more efficient than homoisocitrate | Thermus thermophilus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | about 20times more efficient than homoisocitrate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
additional information | no substrate: 3-isopropylmalate | Thermus thermophilus | ? | - |
? | |
additional information | no substrate: 3-isopropylmalate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | sedimentation equilibrium analysis, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
HICDH | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life 16.7 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
171 | - |
isocitrate | 60°C, pH 8.0 | Thermus thermophilus | |
171 | - |
homoisocitrate | 60°C, pH 8.0 | Thermus thermophilus |