BRENDA - Enzyme Database
show all sequences of 1.1.1.286

Characterization of homoisocitrate dehydrogenase involved in lysine biosynthesis of an extremely thermophilic bacterium, Thermus thermophilus HB27, and evolutionary implication of beta-decarboxylating dehydrogenase

Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Yamane, H.; J. Biol. Chem. 278, 1864-1871 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Thermus thermophilus
Engineering
Amino acid exchange
Commentary
Organism
R85V
complete loss of activity with citrate, retains activity with isocitrate
Thermus thermophilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.405
-
isocitrate
60C, pH 8.0
Thermus thermophilus
7.486
-
homoisocitrate
60C, pH 8.0
Thermus thermophilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus thermophilus
Q72IW9
expression in Escherichia coli
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Q72IW9
expression in Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
homoisocitrate + NAD+
-
656127
Thermus thermophilus
? + NADH
-
-
-
?
homoisocitrate + NAD+
-
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
? + NADH
-
-
-
?
isocitrate + NAD+
about 20times more efficient than homoisocitrate
656127
Thermus thermophilus
2-oxoglutarate + CO2 + NADH
-
-
-
?
isocitrate + NAD+
about 20times more efficient than homoisocitrate
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
2-oxoglutarate + CO2 + NADH
-
-
-
?
additional information
no substrate: 3-isopropylmalate
656127
Thermus thermophilus
?
-
-
-
-
additional information
no substrate: 3-isopropylmalate
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
sedimentation equilibrium analysis, SDS-PAGE
Thermus thermophilus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
90
-
half-life 16.7 h
Thermus thermophilus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
171
-
homoisocitrate
60C, pH 8.0
Thermus thermophilus
171
-
isocitrate
60C, pH 8.0
Thermus thermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Thermus thermophilus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R85V
complete loss of activity with citrate, retains activity with isocitrate
Thermus thermophilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.405
-
isocitrate
60C, pH 8.0
Thermus thermophilus
7.486
-
homoisocitrate
60C, pH 8.0
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
homoisocitrate + NAD+
-
656127
Thermus thermophilus
? + NADH
-
-
-
?
homoisocitrate + NAD+
-
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
? + NADH
-
-
-
?
isocitrate + NAD+
about 20times more efficient than homoisocitrate
656127
Thermus thermophilus
2-oxoglutarate + CO2 + NADH
-
-
-
?
isocitrate + NAD+
about 20times more efficient than homoisocitrate
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
2-oxoglutarate + CO2 + NADH
-
-
-
?
additional information
no substrate: 3-isopropylmalate
656127
Thermus thermophilus
?
-
-
-
-
additional information
no substrate: 3-isopropylmalate
656127
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
sedimentation equilibrium analysis, SDS-PAGE
Thermus thermophilus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
90
-
half-life 16.7 h
Thermus thermophilus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
171
-
homoisocitrate
60C, pH 8.0
Thermus thermophilus
171
-
isocitrate
60C, pH 8.0
Thermus thermophilus
Other publictions for EC 1.1.1.286
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740021
Shimizu
Structure and function of an a ...
Thermococcus kodakarensis, Thermococcus kodakarensis KRD1
Biochem. J.
474
105-122
2017
-
-
1
1
6
-
-
32
-
1
-
4
-
5
-
-
1
-
-
-
-
-
8
1
1
-
-
16
1
-
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
32
-
1
-
4
-
-
-
1
-
-
-
-
8
1
1
-
-
16
1
-
-
-
-
3
3
-
31
31
738195
Takahashi
Characterization of two ?-deca ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius MW001
Extremophiles
20
843-853
2016
-
-
1
-
-
-
-
2
-
-
-
6
-
5
-
-
1
-
-
-
-
-
6
1
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
-
6
-
-
-
1
-
-
-
-
6
1
1
-
-
2
1
-
-
-
-
1
1
-
2
2
740000
Takahashi
Determinants of dual substrate ...
Thermus thermophilus, Thermus thermophilus DSM 7039
Biochem. Biophys. Res. Commun.
478
1688-1693
2016
-
-
1
1
-
-
1
2
-
1
-
6
-
2
-
-
1
-
-
-
-
-
8
1
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
1
-
-
-
1
-
2
-
1
-
6
-
-
-
1
-
-
-
-
8
1
1
-
-
2
1
-
-
-
-
3
3
-
2
2
685232
Lin
Chemical mechanism of homoisoc ...
Saccharomyces cerevisiae
Biochemistry
47
4169-4180
2008
-
-
-
-
-
-
3
1
-
-
-
1
-
1
-
-
-
2
-
-
-
-
3
-
1
-
-
-
1
1
-
1
1
-
-
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
696220
Lin
Potassium is an activator of h ...
Saccharomyces cerevisiae
Biochemistry
47
10809-10815
2008
-
-
-
-
-
-
4
-
-
4
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
1
-
-
-
-
4
3
-
-
4
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660907
Miyazaki
Bifunctional isocitrate-homois ...
Pyrococcus horikoshii
Biochem. Biophys. Res. Commun.
331
341-346
2005
-
-
1
-
-
-
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
660918
Miyazaki
Identification of a novel trif ...
Deinococcus radiodurans
Biochem. Biophys. Res. Commun.
336
596-602
2005
-
-
-
-
4
-
3
8
-
3
3
-
-
1
-
-
-
-
-
-
-
-
5
1
-
-
-
8
-
-
-
1
-
-
-
-
-
-
1
-
4
-
-
3
-
8
-
3
3
-
-
-
-
-
-
-
-
-
5
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
656127
Miyazaki
Characterization of homoisocit ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
J. Biol. Chem.
278
1864-1871
2003
-
-
1
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
1
-
-
1
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
6
1
-
-
1
2
-
-
-
-
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-
-
-
-
-