Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, complete inhibition | [Candida] boidinii | |
Cd2+ | 1 mM, complete inhibition | Komagataella pastoris | |
citrate | 1 mM, 24% inhibition | Komagataella pastoris | |
CN- | - |
[Candida] boidinii | |
CN- | 1 mM, 48% inhibition | Komagataella pastoris | |
Cu2+ | - |
[Candida] boidinii | |
Cu2+ | 1 mM, 76% inhibition | Komagataella pastoris | |
EDTA | 1 mM, 12% inhibition | Komagataella pastoris | |
Hg2+ | - |
[Candida] boidinii | |
Hg2+ | 1 mM, 90% inhibition | Komagataella pastoris | |
NADH | - |
[Candida] boidinii | |
NADH | product inhibition | Komagataella pastoris | |
NO2- | 1 mM, 14% inhibition | Komagataella pastoris | |
Zn2+ | - |
[Candida] boidinii | |
Zn2+ | 1 mM, 28% inhibition | Komagataella pastoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
NAD+ | - |
Komagataella pastoris | |
0.43 | - |
formaldehyde | - |
Komagataella pastoris | |
0.48 | - |
glutathione | - |
Komagataella pastoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Komagataella pastoris |
84000 | - |
gel filtration | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kloeckera sp. | - |
- |
- |
Komagataella pastoris | - |
- |
- |
[Candida] boidinii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.2 | - |
- |
Komagataella pastoris |
Storage Stability | Organism |
---|---|
4°C, in presence of glycerol, 70-80% recovery of activity after 7 days | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
[Candida] boidinii | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Kloeckera sp. | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris IFP 206 | S-formylglutathione + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
[Candida] boidinii |
dimer | 2 * 39000, SDS-PAGE | Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Kloeckera sp. |
35 | - |
- |
[Candida] boidinii |
47 | - |
- |
Komagataella pastoris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | 20°C: about 60% of maximal activity, 65°C: about 40% of maximal activity | Komagataella pastoris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
10 min, 50% loss of activity | Kloeckera sp. |
52 | - |
10 min, 50% loss of activity | Komagataella pastoris |
52 | - |
10 min, 50% loss of activity | [Candida] boidinii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.233 | - |
glutathione | - |
Komagataella pastoris | |
0.267 | - |
formaldehyde | - |
Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Komagataella pastoris |
8 | - |
- |
Komagataella pastoris |
8 | - |
- |
[Candida] boidinii |
8 | - |
- |
Kloeckera sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 9.3 | 50% of maximal activity at pH 6.4 and at pH 9.3 | Komagataella pastoris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
37°C, 10 min, 50% loss of activity | Komagataella pastoris |
7 | - |
37°C, 10 min, about 20% loss of activity | Komagataella pastoris |
8.5 | - |
37°C, 10 min, about 30% loss of activity | Komagataella pastoris |
9.4 | - |
37°C, 10 min, about 50% loss of activity | Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Komagataella pastoris | |
NAD+ | - |
[Candida] boidinii | |
NAD+ | - |
Kloeckera sp. |