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Literature summary for 1.1.1.284 extracted from

  • Ken, C.F.; Huang, C.Y.; Wen, L.; Huang, J.K.; Lin, C.T.
    Modulation of nitrosative stress via glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase (2014), Int. J. Mol. Sci., 15, 14166-14179 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.284

Activating Compound

Activating Compound Comment Organism Structure
glutathione dependent on Taiwanofungus camphoratus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in HEK 293T cells, transfectioon via a v-raf/v-myc oncogene-carrying retrovirus, improvement by codon optimization. The Tc-GFD protein expressed in pcGFD-O transfected 293T cells provides protection to these cells against apoptosis under GSNO induced oxidative stress, overview Taiwanofungus camphoratus

Protein Variants

Protein Variants Comment Organism
C47S site-directed mutagenesis, active site mutant Taiwanofungus camphoratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-(hydroxymethyl)glutathione + NAD+ Taiwanofungus camphoratus
-
 S-formylglutathione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Taiwanofungus camphoratus D2CFJ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-(hydroxymethyl)glutathione + NAD+
-
 Taiwanofungus camphoratus S-formylglutathione + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GFD
-
 Taiwanofungus camphoratus
Glutathione-dependent formaldehyde dehydrogenase
-
 Taiwanofungus camphoratus
Tc-GFD formerly named Ac-GFD Taiwanofungus camphoratus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Taiwanofungus camphoratus

General Information

General Information Comment Organism
physiological function glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. Formaldehyde detoxification involves the conversion of formaldehyde to formate, which consists of three reactions: The first reaction is a spontaneous condensation reaction between formaldehyde and glutathione producing S-hydroxymethylglutathione (HMGSH). The second reaction, catalyzed by enzyme GFD, is the oxidation of HMGSH to form S-formylglutathione. In the third reaction, catalyzed by S-formylglutathione hydrolase (FGH), formate is formed and glutathione regenerated Taiwanofungus camphoratus