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Literature summary for 1.1.1.283 extracted from
- Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae (1985), Eur. J. Biochem., 151, 631-636.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Saccharomyces cerevisiae |  |
| dithiothreitol | activates | Saccharomyces cerevisiae | |
| glutathione | activates | Saccharomyces cerevisiae | |
| L-cysteine | activates | Saccharomyces cerevisiae | |
| Triton X-100 | activates | Saccharomyces cerevisiae | |
| Tween 80 | activates | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Saccharomyces cerevisiae | |
| iodoacetate | - |
Saccharomyces cerevisiae | |
| N-dodecanoylsarcosine | - |
Saccharomyces cerevisiae | |
| N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
| NADP+ | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
| sodium cholate | - |
Saccharomyces cerevisiae | |
| sodium dodecylsulfate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 1.54 | - |
Phenylglyoxal | - |
Saccharomyces cerevisiae | |
| 5.88 | - |
methylglyoxal | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylglyoxal + NADPH | Saccharomyces cerevisiae | similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ | lactaldehyde + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 6.6% carbohydrate, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.38 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxal + NADPH | - |
Saccharomyces cerevisiae | glycolaldehyde + NADP+ | - |
ir | |
| methylglyoxal + NADPH | similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ | Saccharomyces cerevisiae | lactaldehyde + NADP+ | - |
ir | |
| methylglyoxal + NADPH + H+ | - |
Saccharomyces cerevisiae | L-lactaldehyde + NADP+ | - |
r | |
| additional information | NADPH required | Saccharomyces cerevisiae | ? | - |
? | |
| phenylglyoxal + NADPH | - |
Saccharomyces cerevisiae | hydroxyphenylacetaldehyde + NADP+ | - |
? | |
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