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Literature summary for 1.1.1.282 extracted from

  • Gritsunov, A.; Peek, J.; Diaz Caballero, J.; Guttman, D.; Christendat, D.
    Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases (2018), Plant J., 95, 812-822 .
    View publication on PubMed
Search for more literature for 1.1.1.282

Cloned(Commentary)

Cloned (Comment) Organism
gene expression analysis, phylogenetic analysis Nicotiana tabacum
gene expression analysis, phylogenetic analysis Brassica napus
gene expression analysis, phylogenetic analysis Brassica rapa
gene expression analysis, phylogenetic analysis Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
 quinate with NAD+, pH and temperature not specified in the publication Nicotiana tabacum
0.129
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica rapa
0.185
-
 quinate with NAD+, pH and temperature not specified in the publication Solanum lycopersicum
0.185
-
 quinate with NADP+, pH and temperature not specified in the publication Solanum lycopersicum
0.189
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica napus
0.219
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica rapa
0.271
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica napus
0.314
-
 NAD+ with shikimate, pH and temperature not specified in the publication Nicotiana tabacum
0.438
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica napus
0.487
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica rapa
0.519
-
 NAD+ with shikimate, pH and temperature not specified in the publication Solanum lycopersicum
0.519
-
 NADP+ with shikimate, pH and temperature not specified in the publication Solanum lycopersicum
0.565
-
 NAD+ with quinate, pH and temperature not specified in the publication Nicotiana tabacum
0.635
-
 NAD+ with quinate, pH and temperature not specified in the publication Solanum lycopersicum
0.635
-
 NADP+ with quinate, pH and temperature not specified in the publication Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the NADP+-specific DHQD-QDH from this plant contains a predicted chloroplast-targeting peptide Solanum lycopersicum 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
quinate + NAD+ Nicotiana tabacum
-
 3-dehydroquinate + NADH + H+
-
 r
quinate + NAD+ Solanum lycopersicum
-
 3-dehydroquinate + NADH + H+
-
 r
quinate + NADP+ Brassica napus
-
 3-dehydroquinate + NADPH + H+
-
 r
quinate + NADP+ Brassica rapa
-
 3-dehydroquinate + NADPH + H+
-
 r
quinate + NADP+ Solanum lycopersicum
-
 3-dehydroquinate + NADPH + H+
-
 r
shikimate + NAD+ Nicotiana tabacum
-
 3-dehydroshikimate + NADH + H+
-
 r
shikimate + NAD+ Solanum lycopersicum
-
 3-dehydroshikimate + NADH + H+
-
 r
shikimate + NADP+ Brassica napus
-
 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+ Brassica rapa
-
 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+ Solanum lycopersicum
-
 3-dehydroshikimate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-
Brassica rapa
-
-
-
Nicotiana tabacum
-
-
-
Solanum lycopersicum A0A3Q7H2B2
-
-
Solanum lycopersicum A0A3Q7IET9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Solanum lycopersicum
-
fruit
-
 Solanum lycopersicum
-
leaf
-
 Solanum lycopersicum
-
additional information gene expression analysis Nicotiana tabacum
-
additional information gene expression analysis Brassica napus
-
additional information gene expression analysis Brassica rapa
-
additional information the gene for the NAD+-dependent enzyme shows higher expression in the roots in the later stages of Solanum lycopersicum fruit development and especially during fruit ripening. Unique expression profiles imply that QDH genes for NAD+- and NADP+-specific enzymes, gene expression analysis Solanum lycopersicum
-
additional information the NADP+-specific enzyme shows higher expression levels in green tissues, including leaves, green stems and flowers and during the early stages of fruit development, gene expression analysis. The unique expression profiles imply that QDH genes for NAD+- and NADP+-specific enzymes are likely to have distinct physiological roles Solanum lycopersicum
-
root
-
 Solanum lycopersicum
-
stem green Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NAD+-utilizing QDHs are more active with quinate than with shikimate Nicotiana tabacum ?
-
-
additional information NAD+-utilizing QDHs are more active with quinate than with shikimate Solanum lycopersicum ?
-
-
quinate + NAD+
-
 Nicotiana tabacum 3-dehydroquinate + NADH + H+
-
 r
quinate + NAD+
-
 Solanum lycopersicum 3-dehydroquinate + NADH + H+
-
 r
quinate + NADP+
-
 Brassica napus 3-dehydroquinate + NADPH + H+
-
 r
quinate + NADP+
-
 Brassica rapa 3-dehydroquinate + NADPH + H+
-
 r
quinate + NADP+
-
 Solanum lycopersicum 3-dehydroquinate + NADPH + H+
-
 r
shikimate + NAD+
-
 Nicotiana tabacum 3-dehydroshikimate + NADH + H+
-
 r
shikimate + NAD+
-
 Solanum lycopersicum 3-dehydroshikimate + NADH + H+
-
 r
shikimate + NADP+
-
 Brassica napus 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+
-
 Brassica rapa 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+
-
 Solanum lycopersicum 3-dehydroshikimate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
More cf. EC 4.2.1.10 Solanum lycopersicum
NAD+ cofactor-specific QDH
-
 Solanum lycopersicum
NADP+ cofactor-specific QDH
-
 Solanum lycopersicum
NADP+-specific DHQD-QDH
-
 Solanum lycopersicum
QDH
-
 Nicotiana tabacum
QDH
-
 Brassica napus
QDH
-
 Brassica rapa
QDH
-
 Solanum lycopersicum
quinate dehydrogenase
-
 Nicotiana tabacum
quinate dehydrogenase
-
 Brassica napus
quinate dehydrogenase
-
 Brassica rapa
quinate dehydrogenase
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0018
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica rapa
0.335
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica rapa
0.493
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica rapa
0.74
-
 NAD+ with shikimate, pH and temperature not specified in the publication Solanum lycopersicum
3.8
-
 NAD+ with shikimate, pH and temperature not specified in the publication Nicotiana tabacum
7
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica napus
8.5
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica napus
8.6
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica napus
9.4
-
 quinate with NAD+, pH and temperature not specified in the publication Solanum lycopersicum
10.5
-
 NAD+ with quinate, pH and temperature not specified in the publication Solanum lycopersicum
16.2
-
 quinate with NAD+, pH and temperature not specified in the publication Nicotiana tabacum
20.8
-
 NAD+ with quinate, pH and temperature not specified in the publication Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Nicotiana tabacum
NAD+
-
 Solanum lycopersicum
NADH
-
 Nicotiana tabacum
NADH
-
 Solanum lycopersicum
NADP+
-
 Brassica napus
NADP+
-
 Brassica rapa
NADP+
-
 Solanum lycopersicum
NADPH
-
 Brassica napus
NADPH
-
 Brassica rapa
NADPH
-
 Solanum lycopersicum

General Information

General Information Comment Organism
evolution plant QDHs arose directly from bifunctional dehydroquinate dehydratase-shikimate dehydrogenases (DHQD-SDHs) through different convergent evolutionary events, detailed phylogenetic analysis, overview. Eudicot and conifer QDHs arose early in vascular plant evolution whereas Brassicaceae QDHs emerged late, process of recurrent evolution of QDH. This family of proteins independently evolved NAD+ and NADP+ specificity in eudicots. The acquisition of QDH activity by these proteins is accompanied by the inactivation or functional evolution of the DHQD domain, as verified by enzyme activity assays and as reflected in the loss of key DHQD active site residues Nicotiana tabacum
evolution plant QDHs arose directly from bifunctional dehydroquinate dehydratase-shikimate dehydrogenases (DHQD-SDHs) through different convergent evolutionary events, detailed phylogenetic analysis, overview. Eudicot and conifer QDHs arose early in vascular plant evolution whereas Brassicaceae QDHs emerged late, process of recurrent evolution of QDH. This family of proteins independently evolved NAD+ and NADP+ specificity in eudicots. The acquisition of QDH activity by these proteins is accompanied by the inactivation or functional evolution of the DHQD domain, as verified by enzyme activity assays and as reflected in the loss of key DHQD active site residues Brassica napus
evolution plant QDHs arose directly from bifunctional dehydroquinate dehydratase-shikimate dehydrogenases (DHQD-SDHs) through different convergent evolutionary events, detailed phylogenetic analysis, overview. Eudicot and conifer QDHs arose early in vascular plant evolution whereas Brassicaceae QDHs emerged late, process of recurrent evolution of QDH. This family of proteins independently evolved NAD+ and NADP+ specificity in eudicots. The acquisition of QDH activity by these proteins is accompanied by the inactivation or functional evolution of the DHQD domain, as verified by enzyme activity assays and as reflected in the loss of key DHQD active site residues Brassica rapa
evolution plant QDHs arose directly from bifunctional dehydroquinate dehydratase-shikimate dehydrogenases (DHQD-SDHs) through different convergent evolutionary events, detailed phylogenetic analysis, overview. Eudicot and conifer QDHs arose early in vascular plant evolution whereas Brassicaceae QDHs emerged late, process of recurrent evolution of QDH. This family of proteins independently evolved NAD+ and NADP+ specificity in eudicots. The acquisition of QDH activity by these proteins is accompanied by the inactivation or functional evolution of the DHQD domain, as verified by enzyme activity assays and as reflected in the loss of key DHQD active site residues Solanum lycopersicum
additional information only four amino acid residues likely to contribute to specificity for one substrate instead of the other, namely S336, S338, T381 and Y550, all of which would be in the direct vicinity of the quinate C1-hydroxyl. Amino acid S336 has previously been shown by mutational analysis to be critical for shikimate binding. The size of the amino acid side chain at position 381 is a key determinant of substrate specificity Nicotiana tabacum
additional information only four amino acid residues likely to contribute to specificity for one substrate instead of the other, namely S336, S338, T381 and Y550, all of which would be in the direct vicinity of the quinate C1-hydroxyl. Amino acid S336 has previously been shown by mutational analysis to be critical for shikimate binding. The size of the amino acid side chain at position 381 is a key determinant of substrate specificity Brassica napus
additional information only four amino acid residues likely to contribute to specificity for one substrate instead of the other, namely S336, S338, T381 and Y550, all of which would be in the direct vicinity of the quinate C1-hydroxyl. Amino acid S336 has previously been shown by mutational analysis to be critical for shikimate binding. The size of the amino acid side chain at position 381 is a key determinant of substrate specificity Brassica rapa
additional information only four amino acid residues likely to contribute to specificity for one substrate instead of the other, namely S336, S338, T381 and Y550, all of which would be in the direct vicinity of the quinate C1-hydroxyl. Amino acid S336 has previously been shown by mutational analysis to be critical for shikimate binding. The size of the amino acid side chain at position 381 is a key determinant of substrate specificity Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica rapa
0.688
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica rapa
1.43
-
 NAD+ with shikimate, pH and temperature not specified in the publication Solanum lycopersicum
3.82
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica rapa
12.1
-
 NAD+ with shikimate, pH and temperature not specified in the publication Nicotiana tabacum
15.98
-
 NADP+ with quinate, pH and temperature not specified in the publication Brassica napus
16.5
-
 NAD+ with quinate, pH and temperature not specified in the publication Solanum lycopersicum
31.73
-
 NADP+ with shikimate, pH and temperature not specified in the publication Brassica napus
36.8
-
 NAD+ with quinate, pH and temperature not specified in the publication Nicotiana tabacum
44.97
-
 quinate with NAD+, pH and temperature not specified in the publication Brassica napus
50.8
-
 quinate with NAD+, pH and temperature not specified in the publication Solanum lycopersicum
151.4
-
 quinate with NAD+, pH and temperature not specified in the publication Nicotiana tabacum