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Literature summary for 1.1.1.280 extracted from

  • Miya, H.; Kawada, M.; Sugiyama, Y.
    Purification and Characterization of Ethyl 2-Methyl-3-oxobutanoate Reductase from Klebsiella pneumoniae IF03319. (1996), Biosci. Biotechnol. Biochem., 60, 760-764.
    View publication on PubMed

Application

Application Comment Organism
synthesis ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM completely inactivates the enzyme Klebsiella pneumoniae
Fe2+ 1 mM completely inactivates the enzyme Klebsiella pneumoniae
Mn2+ 1 mM completely inactivates the enzyme Klebsiella pneumoniae
Zn2+ 1 mM completely inactivates the enzyme Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6
-
2-methyl-3-oxobutanoate
-
Klebsiella pneumoniae
12.5
-
benzyl 2-methyl-3-oxobutanoate
-
Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Klebsiella pneumoniae
31000
-
1 * 31000, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
IFO3319
-
Klebsiella pneumoniae IFO3319
-
IFO3319
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-oxobutanoate + NADPH + H+
-
Klebsiella pneumoniae (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
-
r
benzyl 2-methyl-3-oxobutanoate + NADPH
-
Klebsiella pneumoniae benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
-
?
benzyl 2-methyl-3-oxobutanoate + NADPH
-
Klebsiella pneumoniae IFO3319 benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
-
?
ethyl 2-methyl-3-oxobutanoate + NADPH
-
Klebsiella pneumoniae ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
-
?
ethyl 2-methyl-3-oxobutanoate + NADPH
-
Klebsiella pneumoniae IFO3319 ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
-
?
ethyl 2-oxocyclohexanecarboxylate + NADPH
-
Klebsiella pneumoniae ethyl (1R,2S)-2-hydroxycyclohexanecarboxylate + NADP+
-
?
ethyl 2-oxocyclopentanecarboxylate + NADPH
-
Klebsiella pneumoniae ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+
-
?
ethyl 2-oxocyclopentanecarboxylate + NADPH
-
Klebsiella pneumoniae IFO3319 ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+
-
?
ethyl 3-oxobutyrate + NADPH
-
Klebsiella pneumoniae ethyl (S)-3-hydroxybutyrate + NADP+
-
?
ethyl 3-oxobutyrate + NADPH
-
Klebsiella pneumoniae IFO3319 ethyl (S)-3-hydroxybutyrate + NADP+
-
?
additional information ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances Klebsiella pneumoniae ?
-
?
additional information ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances Klebsiella pneumoniae IFO3319 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to 45øC, inactivated above 50øC, 10 min Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction reaction Klebsiella pneumoniae
10
-
oxidation reaction Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8
-
Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Klebsiella pneumoniae