Application | Comment | Organism |
---|---|---|
synthesis | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM completely inactivates the enzyme | Klebsiella pneumoniae | |
Fe2+ | 1 mM completely inactivates the enzyme | Klebsiella pneumoniae | |
Mn2+ | 1 mM completely inactivates the enzyme | Klebsiella pneumoniae | |
Zn2+ | 1 mM completely inactivates the enzyme | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
2-methyl-3-oxobutanoate | - |
Klebsiella pneumoniae | |
12.5 | - |
benzyl 2-methyl-3-oxobutanoate | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Klebsiella pneumoniae |
31000 | - |
1 * 31000, SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
IFO3319 | - |
Klebsiella pneumoniae IFO3319 | - |
IFO3319 | - |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-oxobutanoate + NADPH + H+ | - |
Klebsiella pneumoniae | (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | - |
r | |
benzyl 2-methyl-3-oxobutanoate + NADPH | - |
Klebsiella pneumoniae | benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | - |
? | |
benzyl 2-methyl-3-oxobutanoate + NADPH | - |
Klebsiella pneumoniae IFO3319 | benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | - |
? | |
ethyl 2-methyl-3-oxobutanoate + NADPH | - |
Klebsiella pneumoniae | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | - |
? | |
ethyl 2-methyl-3-oxobutanoate + NADPH | - |
Klebsiella pneumoniae IFO3319 | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+ | - |
? | |
ethyl 2-oxocyclohexanecarboxylate + NADPH | - |
Klebsiella pneumoniae | ethyl (1R,2S)-2-hydroxycyclohexanecarboxylate + NADP+ | - |
? | |
ethyl 2-oxocyclopentanecarboxylate + NADPH | - |
Klebsiella pneumoniae | ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+ | - |
? | |
ethyl 2-oxocyclopentanecarboxylate + NADPH | - |
Klebsiella pneumoniae IFO3319 | ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+ | - |
? | |
ethyl 3-oxobutyrate + NADPH | - |
Klebsiella pneumoniae | ethyl (S)-3-hydroxybutyrate + NADP+ | - |
? | |
ethyl 3-oxobutyrate + NADPH | - |
Klebsiella pneumoniae IFO3319 | ethyl (S)-3-hydroxybutyrate + NADP+ | - |
? | |
additional information | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances | Klebsiella pneumoniae | ? | - |
? | |
additional information | ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances | Klebsiella pneumoniae IFO3319 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to 45øC, inactivated above 50øC, 10 min | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction reaction | Klebsiella pneumoniae |
10 | - |
oxidation reaction | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Klebsiella pneumoniae |