Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline 5-sulfonic acid | - |
Saccharomyces cerevisiae | |
dipicolinate | - |
Saccharomyces cerevisiae | |
HgCl2 | - |
Saccharomyces cerevisiae | |
o-phenanthroline | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
ethyl 3-oxobutyrate | - |
Saccharomyces cerevisiae | |
5.3 | - |
ethyl 3-oxohexanoate | - |
Saccharomyces cerevisiae | |
13.1 | - |
5-Oxohexanoate | - |
Saccharomyces cerevisiae | |
17.1 | - |
4-oxopentanoate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
2 * 24000, SDS-PAGE | Saccharomyces cerevisiae |
48000 | 50000 | gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.411 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-oxopentanoate + NADPH | - |
Saccharomyces cerevisiae | (S)-4-hydroxypentanoate + NADP+ | - |
? | |
5-oxohexanoate + NADPH | - |
Saccharomyces cerevisiae | (S)-5-hydroxyhexanoate + NADP+ | - |
? | |
ethyl 3-oxobutyrate + NADPH | - |
Saccharomyces cerevisiae | ethyl (S)-3-hydroxybutyrate + NADP+ | - |
? | |
ethyl 3-oxohexanoate + NADPH | - |
Saccharomyces cerevisiae | ethyl (S)-3-hydroxyhexanoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Saccharomyces cerevisiae |