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Literature summary for 1.1.1.276 extracted from

  • Fujisawa, H.; Nagata, S.; Chowdhury, E.K.; Matsumoto, M.; Misono, H.
    Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens (2002), Biosci. Biotechnol. Biochem., 66, 1137-1139.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression in Escherichia coli JM109 Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + NADP+ Escherichia coli
-
 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+ Agrobacterium tumefaciens
-
 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+ Agrobacterium tumefaciens ICR 1600
-
 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+ Escherichia coli JM109
-
 2-aminomalonate semialdehyde + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens ICR 1600
-
-
-
Escherichia coli
-
-
-
Escherichia coli JM109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli, to homogeneity Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
-
 Escherichia coli
0.043
-
 recombinant enzyme in Escherichia coli Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + NADP+
-
 Escherichia coli 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+
-
 Agrobacterium tumefaciens 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+ enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism Agrobacterium tumefaciens 2-aminomalonate semialdehyde + NADPH 2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde ?
L-serine + NADP+
-
 Agrobacterium tumefaciens ICR 1600 2-aminomalonate semialdehyde + NADPH
-
 ?
L-serine + NADP+ enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism Agrobacterium tumefaciens ICR 1600 2-aminomalonate semialdehyde + NADPH 2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde ?
L-serine + NADP+
-
 Escherichia coli JM109 2-aminomalonate semialdehyde + NADPH
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Escherichia coli
tetramer x * 25000-26600, recombinant enzyme, SDS-PAGE and amino acid sequence determination Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli
30
-
 assay at Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Escherichia coli
NADP+ dependent on Agrobacterium tumefaciens