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Literature summary for 1.1.1.273 extracted from
- Vellosimine reductase. A specific enzyme involved in the cell-free biosynthesis of sarpagine type alkaloids (1983), Tetrahedron Lett., 24, 1695-1698.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0167 | - |
vellosimine | - |
Rauvolfia serpentina |  |
| 0.02 | - |
NADPH | - |
Rauvolfia serpentina | |
| 0.11 | - |
geissoschizal | - |
Rauvolfia serpentina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vellosimine + NADPH | Rauvolfia serpentina | specific for NADPH, may be involved in the conversion of sarpagine type alkaloids | 10-deoxysarpagine + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, hydroxylapatite, Matrex gel Green A | Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Rauvolfia serpentina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00096 | - |
geissoschizal reduction | Rauvolfia serpentina |
| 0.0012 | - |
vellosimine reduction | Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geissoschizal + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| vellosimine + NADPH | specific for NADPH | Rauvolfia serpentina | 10-deoxysarpagine + NADP+ | - |
? | |
| vellosimine + NADPH | specific for NADPH, may be involved in the conversion of sarpagine type alkaloids | Rauvolfia serpentina | 10-deoxysarpagine + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rauvolfia serpentina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rauvolfia serpentina | |
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Release 2023.1 (January 2023)
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