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Literature summary for 1.1.1.271 extracted from

  • Zhou, H.; Sun, L.; Li, J.; Xu, C.; Yu, F.; Liu, Y.; Ji, C.; He, J.
    The crystal structure of human GDP-L-fucose synthase (2013), Acta Biochim. Biophys. Sin. (Shanghai), 45, 720-725.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene fx, expression of the N-terminally His6-tagged enzyme in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapor diffusion method, 5 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM NaCl, is mixed with well solution containing 100 mM Tris, pH 8.0, 30% PEG monomethyl ether 2000, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular replacement Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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gene fx
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Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 Homo sapiens GDP-beta-L-fucose + NADP+
-
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Subunits

Subunits Comment Organism
tetramer two homodimers, each subunit consists of two domains, a Rossmann-fold NADPH-binding motif and a carboxyl terminal domain, quaternary structure and intermolecular contacts, overview Homo sapiens

Synonyms

Synonyms Comment Organism
FX protein
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on, binding site structure, overview Homo sapiens