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Literature summary for 1.1.1.270 extracted from

  • Mythen, S.; Devendran, S.; Méndez-García, C.; Cann, I.; Ridlon, J.
    Targeted synthesis and characterization of a gene cluster encoding NAD(P)H-dependent 3alpha-, 3beta-, and 12alpha-hydroxysteroid dehydrogenases from Eggerthella CAG 298, a gut metagenomic sequence (2018), Appl. Environ. Microbiol., 84, e02475-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.270

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli Eggerthella sp. CAG:298

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.283
-
 isodeoxycholic acid coenzyme: NADP+, pH 9.5, temperature not specified in the publication Eggerthella sp. CAG:298
0.47
-
 3-oxodeoxycholic acid coenzyme: NADPH, pH 6.5, temperature not specified in the publication Eggerthella sp. CAG:298

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31590
-
 calculation from sequence Eggerthella sp. CAG:298

Organism

Organism UniProt Comment Textmining
Eggerthella sp. CAG:298 R7AF73
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Eggerthella sp. CAG:298

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxocholic acid + NAD(P)H + H+ 10.3% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 cholic acid + NAD(P)+
-
 r
3-oxodeoxycholic acid + NAD(P)H + H+ 55.2% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 isodeoxycholic acid + NAD(P)+
-
 r
chenodeoxycholic acid + NAD(P)+ 11.7% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-chenodeoxycholic acid + NAD(P)H + H+
-
 r
cholic acid + NAD(P)+ 12.7% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-cholic acid + NAD(P)H + H+
-
 r
deoxycholic acid + NAD(P)+ 22.4% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-deoxycholic acid + NAD(P)H + H+
-
 r
glycochenodeoxycholic acid + NAD(P)+ 13.2% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
glycocholic acid + NAD(P)+ 12.4% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
glycodeoxycholic acid + NAD(P)+ 20.6% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
isodeoxycholic acid + NADP+
-
 Eggerthella sp. CAG:298 3-oxodeoxycholic acid + NADPH + H+
-
 r
taurochenodeoxycholic acid + NAD(P)+ 11.5% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
taurocholic acid + NAD(P)+ 11.5% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
taurodeoxychloic acid + NAD(P)+ 21.8% of the activity compared to the substrate isodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r

Subunits

Subunits Comment Organism
? x * 31590, calculation from sequence Eggerthella sp. CAG:298

Synonyms

Synonyms Comment Organism
3beta-HSDH
-
 Eggerthella sp. CAG:298
CDD59473
-
 Eggerthella sp. CAG:298

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 3-oxodeoxycholic acid coenzyme: NADPH, pH 6.5, temperature not specified in the publication Eggerthella sp. CAG:298
0.022
-
 isodeoxycholic acid coenzyme: NADP+, pH 9.5, temperature not specified in the publication Eggerthella sp. CAG:298

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reductive reaction Eggerthella sp. CAG:298
9 10 oxidative reaction Eggerthella sp. CAG:298

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Eggerthella sp. CAG:298
NADPH
-
 Eggerthella sp. CAG:298

General Information

General Information Comment Organism
physiological function enzyme in an iso-bile acid pathway Eggerthella sp. CAG:298

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.015
-
 3-oxodeoxycholic acid coenzyme: NADPH, pH 6.5, temperature not specified in the publication Eggerthella sp. CAG:298
0.078
-
 isodeoxycholic acid coenzyme: NADP+, pH 9.5, temperature not specified in the publication Eggerthella sp. CAG:298