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Literature summary for 1.1.1.27 extracted from

  • Yao, S.; Mikkelsen, M.J.
    Metabolic engineering to improve ethanol production in Thermoanaerobacter mathranii (2010), Appl. Microbiol. Biotechnol., 88, 199-208.
    View publication on PubMed

Application

Application Comment Organism
synthesis Thermoanaerobacter mathranii can produce ethanol from lignocellulosic biomass at high temperatures. Deletion of the Ldh gene coding for lactate dehydrogenase eliminates an NADH oxidation pathway. To further facilitate NADH regeneration used for ethanol formation, a heterologous gene GldA encoding an NAD+-dependent glycerol dehydrogenase is expressed leading to increased ethanol yield in the presence of glycerol using xylose as a substrate. The metabolism of the cells is shifted toward the production of ethanol over acetate, hence restoring the redox balance. The recombinant enzyme acquired the capability to utilize glycerol as an extra carbon source in the presence of xylose resulting in a higher ethanol yield Thermoanaerobacter mathranii

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter mathranii
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Thermoanaerobacter mathranii BG1
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