Application | Comment | Organism |
---|---|---|
synthesis | the enzyme might be useful in the production of phenyllactate | Lactobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
pyruvate | pH 6.5, 30°C, purified enzyme | Lactobacillus sp. | |
1.76 | - |
phenylpyruvate | pH 6.5, 30°C, purified enzyme | Lactobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Lactobacillus sp. |
78000 | - |
gel filtration | Lactobacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Lactobacillus sp. | - |
pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | Lactobacillus sp. SK007 | - |
pyruvate + NADH + H+ | - |
r | |
phenylpyruvate + NADH | Lactobacillus sp. | - |
phenyllactate + NAD+ | - |
r | |
phenylpyruvate + NADH | Lactobacillus sp. SK007 | - |
phenyllactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus sp. | - |
- |
- |
Lactobacillus sp. SK007 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 34.3fold to about 98% purity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Lactobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
203.3 | - |
purified native enzyme, substrate pyruvate | Lactobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Lactobacillus sp. | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Lactobacillus sp. SK007 | pyruvate + NADH + H+ | - |
r | |
phenylpyruvate + NADH | - |
Lactobacillus sp. | phenyllactate + NAD+ | - |
r | |
phenylpyruvate + NADH | - |
Lactobacillus sp. SK007 | phenyllactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Lactobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Lactobacillus sp. |
LDH | - |
Lactobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
with substrate phenylpyruvate | Lactobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 90% of maximal activity at 50°C and 50% at 60°C | Lactobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
with substrate phenylpyruvate | Lactobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, the enzyme is inactive below pH 3.0 and above pH 9.0 | Lactobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | rather stable from pH 4.0 to pH 8.0, but rapid decline in activity below pH 4.0 and above pH 8.0 | Lactobacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lactobacillus sp. | |
NADH | - |
Lactobacillus sp. |