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Literature summary for 1.1.1.27 extracted from

  • Steinbuechel, A.; Schlegel, H.G.
    NAD-linked L(+)-lactate dehydrogenase from the strict aerobe Alcaligenes eutrophus. 1. Purification and properties (1983), Eur. J. Biochem., 130, 321-328.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate not dependent on fructose 1,6-diphosphate Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
3-Fluoropyruvate
-
Cupriavidus necator
Ag+
-
Cupriavidus necator
Cd2+ partial Cupriavidus necator
Cu2+
-
Cupriavidus necator
Hg2+
-
Cupriavidus necator
NAD+
-
Cupriavidus necator
Ni2+ partial Cupriavidus necator
oxalate
-
Cupriavidus necator
oxaloacetate
-
Cupriavidus necator
oxamate
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cupriavidus necator
phosphoenolpyruvate
-
Cupriavidus necator
Zn2+
-
Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Cupriavidus necator
65000 67000 gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
824.6
-
-
Cupriavidus necator

Storage Stability

Storage Stability Organism
-20°C, stable for months Cupriavidus necator
stabilized for storage at 4°C by addition of 0.2-1 M potassium chloride or 3.2 M ammonium sulfate Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Cupriavidus necator pyruvate + NADH + H+
-
r
2-oxobutyrate + NADH 107% of the activity with pyruvate Cupriavidus necator 2-hydroxybutyrate + NAD+
-
?
2-oxoglutarate + NADH + H+ 79.6% of the activity with pyruvate Cupriavidus necator 2-hydroxyglutarate + NAD+
-
?
2-oxopentanoate + NADH 125% of the activity with pyruvate Cupriavidus necator 2-hydroxypentanoate + NAD+
-
?
3-fluoropyruvate + NADH 33.6% of the activity with pyruvate Cupriavidus necator ?
-
?
3-methyl-2-oxobutanoate + NADH 28.5% of the activity with pyruvate Cupriavidus necator 2-hydroxy-3-methylbutanoate + NAD+
-
?
3-methyl-2-oxopentanoate + NADH 5.3% of the activity with pyruvate Cupriavidus necator 2-hydroxy-3-methylpentanoate + NAD+
-
?
4-methyl-2-oxopentanoate + NADH 39% of the activity with pyruvate Cupriavidus necator 2-hydroxy-4-methylpentanoate + NAD+
-
?
pyruvate + NADH + H+
-
Cupriavidus necator (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42 45
-
Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of pyruvate Cupriavidus necator
9.8
-
oxidation of lactate Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5.3 8 pH 5.3: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, reduction of pyruvate Cupriavidus necator
8.5 10.7 pH 8.5: about 60% of maximal activity, pH 10.7: about 70% of maximal activity, oxidation of lactate Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Cupriavidus necator
NADH coenzyme Cupriavidus necator