Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | dependent on | Streptococcus uberis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADH | - |
Streptococcus uberis | |
0.23 | - |
NAD+ | - |
Streptococcus uberis | |
1.75 | - |
pyruvate | - |
Streptococcus uberis | |
109 | - |
(S)-lactate | - |
Streptococcus uberis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35900 | - |
4 * 35900, SDS-PAGE | Streptococcus uberis |
144000 | - |
equilibrium sedimentation | Streptococcus uberis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus uberis | - |
fructose 1,6-diphosphate dependent enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus uberis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1422 | - |
- |
Streptococcus uberis |
Storage Stability | Organism |
---|---|
-5°C, precipitated from phosphate buffer with (NH4)2SO4 and storage in the supernatant solution, stable for several weeks | Streptococcus uberis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Streptococcus uberis | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | - |
Streptococcus uberis | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35900, SDS-PAGE | Streptococcus uberis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptococcus uberis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 8 | no activity below pH 4.3 and above pH 8.0 | Streptococcus uberis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Streptococcus uberis | |
NADH | coenzyme | Streptococcus uberis |