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Literature summary for 1.1.1.267 extracted from

  • You, M.K.; Lee, Y.J.; Kim, J.K.; Baek, S.A.; Jeon, Y.A.; Lim, S.H.; Ha, S.H.
    The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds (2020), BMC Plant Biol., 20, 167 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
gene DXR, phylogenetic analysis and tree, recombinant overexpression of DXR in Oryza sativa leaves and seeds, quantitative real-time PCR enzyme expression analysis Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information expression profiles of carotenoid biosynthetic genes in leaves and seeds among three independent transgenic plants for each OsDXS2 and OsDXR construct relative to non-transgenic (NT) and stPAC plants. The endogenous expression of OsDXS1 and OsDXS2 increases up to 6.7fold and 4.0fold, respectively, following the overexpression of OsDXR, but the enhanced activity of OsDXS2 does not cause a significant increase in OsDXR expression, even though the expression of the OsPSY2 gene is significantly increased up to 5.7fold following the overexpression of OsDXS2 Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Oryza sativa Japonica Group 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Oryza sativa Japonica Group
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q8W250 single gene; cv. Ilmi
-

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Oryza sativa Japonica Group
-
leaf OsDXR is strongly expressed in the leaves of both developmental stages, high overall expression level in leaves Oryza sativa Japonica Group
-
additional information enzyme expression analysis in in leaves and roots of the vegetative stage and in leaves, roots, florets, and seeds of the reproductive stage, expression patterns, overview Oryza sativa Japonica Group
-
root
-
 Oryza sativa Japonica Group
-
seed
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Oryza sativa Japonica Group 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
More OsDXR has three highly conserved domains, including a proline-rich domain, two NADPH binding sites, and two substrate-binding sites Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
deoxyxylulose 5-phosphate reductoisomerase
-
 Oryza sativa Japonica Group
DXP reductoisomerase
-
 Oryza sativa Japonica Group
DXR
-
 Oryza sativa Japonica Group
Os01g01710
-
 Oryza sativa Japonica Group
OsDXR
-
 Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Oryza sativa Japonica Group
NADPH
-
 Oryza sativa Japonica Group

General Information

General Information Comment Organism
evolution phylogenetic analysis, rice DXR is encoded by the single copy gene OsDXR and phylogenetically categorized into plant DXR clade II, which includes the tree plants gingko, yew, and pine, and the Poaceae family plants sorghum (Sorghum bicolor), maize, foxtail millet, and barley (Hordeum vulgare). On the other hand, rice DXR is not categorized into clade I, which includes herbage plants Oryza sativa Japonica Group
malfunction the overexpression of each OsDXS2 or OsDXR causes no positive effect on the accumulation of either carotenoids or chlorophylls in leaves and seeds, but overexpression of either OsDXS2 or OsDXR affects seed carotenoid metabolism, total carotenoid content is increased by 26% in the PGD1::OsDXS2 lines and decreased by 11% in the PGD1::OsDXR lines, overview. The endogenous expression of OsDXS1 and OsDXS2 increases up to 6.7fold and 4.0fold, respectively, following the overexpression of OsDXR, but the enhanced activity of OsDXS2 does not cause a significant increase in OsDXR expression, even though the expression of the OsPSY2 gene is significantly increased up to 5.7fold following the overexpression of OsDXS2 Oryza sativa Japonica Group
metabolism organ-specific differential roles of rice Deoxyxylulose 5-phosphate synthase (DXS) and deoxyxylulose 5-phosphate reductoisomerase (DXR), the first two enzymes of the methylerythritol 4-phosphate (MEP) pathway, in carotenoid metabolism in Oryza sativa leaves and seeds. DXS and DXR are the enzymes that catalyze the first two enzyme steps of the MEP pathway to supply the isoprene building-blocks of carotenoids. Enzyme OsDXS2 functions as a rate-limiting enzyme supplying IPP/DMAPPs to seed carotenoid metabolism, but OsDXR does not in either leaves or seeds. Carotenoid and MEP pathway regulation, overview Oryza sativa Japonica Group
physiological function enzyme OsDXS2 functions as a rate-limiting enzyme supplying IPP/DMAPPs to seed carotenoid metabolism, but OsDXR does not in either leaves or seeds. DXR plays essential roles in the development and survival of plants. No positive effect of OsDXR on carotenoid and chlorophyll metabolism in rice leaves. OsDXR-mediated transcriptional alteration of intrinsic carotenogenetic genes in rice leaves and seeds, overview Oryza sativa Japonica Group