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Literature summary for 1.1.1.267 extracted from

  • Sharma, R.; Devi, K.; Modi, M.K.; Sen, P.
    In silico characterization and differential expression analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) of Centella asiatica (2021), 3 Biotech, 11, 184 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene DXR, sequence comparisons and phylogenetic analysis and tree, semi-quantitative RT-PCR and quantitative real-time PCR expression analysis, recombinant expression Centella asiatica

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme sequence contains a conserved transit peptide for plastid motif Cys-Ser-(Ala/Met/Val/Thr) Centella asiatica 9507
-
additional information Centella DXR transcript is localized in chloroplasts and other plastids Centella asiatica
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Centella asiatica
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Centella asiatica A0A0A6Z621
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Centella asiatica
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additional information CaDXR is differentially expressed in different tissues with maximal expression in the node and lowest in the roots, Centella DXR transcript is expressed in vegetative tissues Centella asiatica
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node high expression level Centella asiatica
-
root low expression level Centella asiatica
-
stem
-
 Centella asiatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Centella asiatica 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 51500, SDS-PAGE Centella asiatica
More enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. CaDXR is a basically alpha-helical protein which contains 21 alpha-helixes, 11 beta-sheets, and one hairpin structure Centella asiatica

Synonyms

Synonyms Comment Organism
CaDXR
-
 Centella asiatica
DXR
-
 Centella asiatica

Cofactor

Cofactor Comment Organism Structure
additional information cofactor docking study with DXR, presence of conserved NADPH-binding motif (GSTGSIGT and LAAGSNV) in N-terminal end of the CaDXR protein sequence Centella asiatica
NADP+
-
 Centella asiatica
NADPH
-
 Centella asiatica

pI Value

Organism Comment pI Value Maximum pI Value
Centella asiatica isoelectric focusing
-
 6.33

General Information

General Information Comment Organism
metabolism the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is the rate-limiting enzyme in the methylerythritol 4-phosphate pathway (MEP) via conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP Centella asiatica
additional information enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. Three-dimensional molecular modeling and model validation, and molecular dynamics simulation of modeled DXR, overview Centella asiatica
physiological function the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), an NADPH-dependent reductase, plays a pivotal role in the methylerythritol 4-phosphate pathway (MEP), in the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP. Structure-function analysis of DXR and regulatory mechanism, molecular dynamics simulations, overview Centella asiatica