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Literature summary for 1.1.1.267 extracted from

  • Kholodar, S.A.; Tombline, G.; Liu, J.; Tan, Z.; Allen, C.L.; Gulick, A.M.; Murkin, A.S.
    Alteration of the flexible loop in 1-deoxy-D-xylulose-5-phosphate reductoisomerase boosts enthalpy-driven inhibition by fosmidomycin (2014), Biochemistry, 53, 3423-3431.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
W203A complete loss of activity Mycobacterium tuberculosis
W203F turnover is largely limited by product release for the wild-type enzyme, chemistry is significantly more rate-limiting for mutants W203F and W203Y. Mutant is more sensitive to fosmidomycin than wild-type. Mutation strongly tips the entropy-enthalpy balance of total binding energy Mycobacterium tuberculosis
W203G complete loss of activity Mycobacterium tuberculosis
W203Y turnover is largely limited by product release for the wild-type enzyme, chemistry is significantly more rate-limiting for mutants W203F and W203Y. Mutant is more sensitive to fosmidomycin than wild-type. Mutation strongly tips the entropy-enthalpy balance of total binding energy Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
fosmidomycin
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
 1-deoxy-D-xylulose 5-phosphate wild-type, pH 7.5, 25°C Mycobacterium tuberculosis
0.39
-
 1-deoxy-D-xylulose 5-phosphate mutant W203Y, pH 7.5, 25°C Mycobacterium tuberculosis
1.015
-
 1-deoxy-D-xylulose 5-phosphate mutant W203F, pH 7.5, 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNS1
-
-
Mycobacterium tuberculosis H37Rv P9WNS1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.59
-
 1-deoxy-D-xylulose 5-phosphate mutant W203F, pH 7.5, 25°C Mycobacterium tuberculosis
3.54
-
 1-deoxy-D-xylulose 5-phosphate mutant W203Y, pH 7.5, 25°C Mycobacterium tuberculosis
4.2
-
 1-deoxy-D-xylulose 5-phosphate wild-type, pH 7.5, 25°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000014
-
 fosmidomycin mutant W203Y, pH 7.5, 25°C Mycobacterium tuberculosis
0.00006
-
 fosmidomycin mutant W203F, pH 7.5, 25°C Mycobacterium tuberculosis
0.000093
-
 fosmidomycin wild-type, pH 7.5, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.46
-
 1-deoxy-D-xylulose 5-phosphate mutant W203F, pH 7.5, 25°C Mycobacterium tuberculosis
9.1
-
 1-deoxy-D-xylulose 5-phosphate mutant W203Y, pH 7.5, 25°C Mycobacterium tuberculosis
31
-
 1-deoxy-D-xylulose 5-phosphate wild-type, pH 7.5, 25°C Mycobacterium tuberculosis