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Literature summary for 1.1.1.267 extracted from
- Pre-steady-state kinetic analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Mycobacterium tuberculosis reveals partially rate-limiting product release by parallel pathways (2012), Biochemistry, 51, 5307-5319.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | because the enzyme is absent in humans, DXR is a target for drug discovery | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues, the mutant is more stable and kinetically similar to the wild-type enzyme | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | product inhibition | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | transient kinetics and kinetic mechanism, stopped-flow fluorescence measurements, overview. Kinetics of pre-steady-state reactions and steady-state kinetics, comparison of simulated and experimental values, burst kinetics model for pre-steady-state kinetics | Mycobacterium tuberculosis | |
| 0.0098 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
| 0.084 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 0.115 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 0.42 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| no activity in Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | random substrate binding and ordered release of NADP+ followed by partially rate-limiting release of 2-C-methyl-D-erythritol 4-phosphate, transient generation of MtDXR-NADPH-MEP ternary complex, kinetic analysis and modeling, overview | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
| 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | NADPH and 2-C-methyl-D-erythritol 4-phosphate bind to the free enzyme and the two bind together to generate a nonproductive ternary complex | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-deoxy-D-xylulose-5-phosphate reductoisomerase | - |
Mycobacterium tuberculosis |
| DXP reductoisomerase | - |
Mycobacterium tuberculosis |
| DXR | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 0.95 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
| 5.25 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 5.25 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.7 | - |
NADP+ | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
| 11.3 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 46 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 540 | - |
NADPH | pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate | Mycobacterium tuberculosis | |
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