Application | Comment | Organism |
---|---|---|
drug development | Escherichia coli Dxr represents a valuable model enzyme for the screening for new antimalarial compounds, since work with the Plasmodium falciparum Dxr is associated with considerably high effort due to the instability of this enzyme and the low yield of the available recombinant expression system, no major differences in the IC50 between Escherichia coli Dxr and Plasmodium falciparum Dxr | Escherichia coli |
drug development | Escherichia coli Dxr represents a valuable model enzyme for the screening for new antimalarial compounds, since work with the Plasmodium falciparum Dxr is associated with considerably high effort due to the instability of this enzyme and the low yield of the available recombinant expression system, no major differences in the IC50 between Escherichia coli Dxr and Plasmodium falciparum Dxr | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
dxr gene amplified and inserted into the pQE9 expression vector resulting in a protein with an amino-terminal His6-tag | Escherichia coli |
into the pQE31 expression vector resulting in an amino-terminal His6 fusion | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3S,4R)-3,4-dihydroxy-4-methyl-5-oxohexylphosphonic acid | - |
Escherichia coli | |
3-(hydroxy([(2-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Escherichia coli | |
3-(hydroxy([(2-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Plasmodium falciparum | |
3-(hydroxy([(3-methylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Escherichia coli | |
3-(hydroxy([(3-methylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Plasmodium falciparum | |
3-(hydroxy([(4-phenoxybutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Escherichia coli | |
3-(hydroxy([(4-phenoxybutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Plasmodium falciparum | |
3-(hydroxy([(4-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Escherichia coli | |
3-(hydroxy([(4-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | - |
Plasmodium falciparum | |
3-([(1H-indol-3-yl)acetyl]amino)propylphosphonic acid | - |
Escherichia coli | |
3-([2-(methoxycarbonyl)benzoyl]amino)propylphosphonic acid | - |
Escherichia coli | |
3-([3-(1H-indol-3-yl)propanoyl]amino)propylphosphonic acid | - |
Escherichia coli | |
3-([4-(1H-indol-3-yl)butanoyl]amino)propylphosphonic acid | - |
Escherichia coli | |
3-[(([(3,4-dimethoxyphenyl)acetyl]amino)acetyl)(hydroxy)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[(([(3,4-dimethoxyphenyl)acetyl]amino)acetyl)(hydroxy)amino]propylphosphonic acid | - |
Plasmodium falciparum | |
3-[(2-hydroxybenzoyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[(3,4-diethoxybenzoyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[(3,4-dimethoxybenzoyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[(4-methylpentanoyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[(4-phenoxybenzoyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[([(cyclopropylcarbonyl)amino]acetyl)(hydroxy)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[([(cyclopropylcarbonyl)amino]acetyl)(hydroxy)amino]propylphosphonic acid | - |
Plasmodium falciparum | |
3-[hydroxy(([3-(trifluoromethoxy)benzoyl]amino)acetyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[hydroxy(([3-(trifluoromethoxy)benzoyl]amino)acetyl)amino]propylphosphonic acid | - |
Plasmodium falciparum | |
3-[hydroxy(([4-(1H-indol-3-yl)butanoyl]amino)acetyl)amino]propylphosphonic acid | - |
Escherichia coli | |
3-[hydroxy(([4-(1H-indol-3-yl)butanoyl]amino)acetyl)amino]propylphosphonic acid | - |
Plasmodium falciparum | |
fosmidomycin | - |
Escherichia coli | |
fosmidomycin | - |
Plasmodium falciparum | |
FR-900098 | fosmidomycin homologue | Escherichia coli | |
FR-900098 | fosmidomycin homologue | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by immobilized metal affinity chromatography on a Co column, ca. 90% pure | Plasmodium falciparum |
by immobilized metal affinity chromatography on a Co column, followed by anion exchange chromatography, gel filtration and ultrafiltration | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C | Escherichia coli |
-70°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Plasmodium falciparum | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DXR | - |
Escherichia coli |
DXR | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli | |
NADPH | - |
Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000018 | - |
- |
Plasmodium falciparum | FR-900098 | |
0.000032 | - |
- |
Plasmodium falciparum | fosmidomycin | |
0.00005 | - |
- |
Escherichia coli | fosmidomycin | |
0.000051 | - |
- |
Escherichia coli | FR-900098 | |
0.0004 | - |
- |
Plasmodium falciparum | 3-(hydroxy([(4-phenoxybutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.001 | - |
- |
Escherichia coli | 3-(hydroxy([(4-phenoxybutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.0029 | - |
- |
Plasmodium falciparum | 3-[hydroxy(([3-(trifluoromethoxy)benzoyl]amino)acetyl)amino]propylphosphonic acid | |
0.0033 | - |
- |
Plasmodium falciparum | 3-[([(cyclopropylcarbonyl)amino]acetyl)(hydroxy)amino]propylphosphonic acid | |
0.0051 | - |
- |
Escherichia coli | 3-[hydroxy(([3-(trifluoromethoxy)benzoyl]amino)acetyl)amino]propylphosphonic acid | |
0.0066 | - |
- |
Plasmodium falciparum | 3-[hydroxy(([4-(1H-indol-3-yl)butanoyl]amino)acetyl)amino]propylphosphonic acid | |
0.0071 | - |
- |
Escherichia coli | 3-[([(cyclopropylcarbonyl)amino]acetyl)(hydroxy)amino]propylphosphonic acid | |
0.0093 | - |
- |
Plasmodium falciparum | 3-(hydroxy([(2-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.0096 | - |
- |
Plasmodium falciparum | 3-(hydroxy([(3-methylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.012 | - |
- |
Escherichia coli | 3-(hydroxy([(3-methylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.012 | - |
- |
Plasmodium falciparum | 3-[(([(3,4-dimethoxyphenyl)acetyl]amino)acetyl)(hydroxy)amino]propylphosphonic acid | |
0.014 | - |
- |
Escherichia coli | 3-[(([(3,4-dimethoxyphenyl)acetyl]amino)acetyl)(hydroxy)amino]propylphosphonic acid | |
0.017 | - |
- |
Escherichia coli | 3-[hydroxy(([4-(1H-indol-3-yl)butanoyl]amino)acetyl)amino]propylphosphonic acid | |
0.019 | - |
- |
Escherichia coli | 3-(hydroxy([(4-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.02 | - |
- |
Escherichia coli | 3-(hydroxy([(2-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.02 | - |
- |
Plasmodium falciparum | 3-(hydroxy([(4-phenylbutanoyl)amino]acetyl)amino)propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | (3S,4R)-3,4-dihydroxy-4-methyl-5-oxohexylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-([4-(1H-indol-3-yl)butanoyl]amino)propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-([3-(1H-indol-3-yl)propanoyl]amino)propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-([(1H-indol-3-yl)acetyl]amino)propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-([2-(methoxycarbonyl)benzoyl]amino)propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-[(2-hydroxybenzoyl)amino]propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-[(3,4-diethoxybenzoyl)amino]propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-[(4-methylpentanoyl)amino]propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-[(4-phenoxybenzoyl)amino]propylphosphonic acid | |
0.03 | - |
- |
Escherichia coli | 3-[(3,4-dimethoxybenzoyl)amino]propylphosphonic acid |