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Literature summary for 1.1.1.265 extracted from

  • Van Nedervelde, L.; Verlingen, V.; Philipp, D.; Debourg, A.
    Purificationa nd characterization of yeast 3-methyl butanal reductases involved in the removal of wort carbonyls during fermentation (1997), Proc. Congr. Eur. Brew. Conv., 26, 447-454.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 6% inhibition at 10 mM Saccharomyces cerevisiae
Ca2+ 9% inhibition at 0.1 mM Saccharomyces cerevisiae
dithiothreitol 35% inhibition at 5 mM Saccharomyces cerevisiae
glutathione 24% inhibition at 5 mM Saccharomyces cerevisiae
KCl 16% inhibition at 0.2 M Saccharomyces cerevisiae
Mg2+ 6% inhibition at 0.1 mM Saccharomyces cerevisiae
NaCl 19% inhibition at 0.2 M Saccharomyces cerevisiae
quercetin 78% inhibition at 0.1 mM Saccharomyces cerevisiae
sodium valporate 5% inhibition at 1 mM Saccharomyces cerevisiae
Zn2+ 28% inhibition at 0.1 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methylbutanal + NAD(P)H Saccharomyces cerevisiae
-
3-methylbutanol + NAD(P)+
-
r
acetaldehyde + NADPH + H+ Saccharomyces cerevisiae
-
ethanol + NADP+
-
r
gluconate + NAD(P)H Saccharomyces cerevisiae very low activity in both reduction and oxidation D-glucose + NAD(P)+
-
r
glyceraldehyde + NAD(P)H Saccharomyces cerevisiae
-
glycerol + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.29
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylbutanal + NAD(P)H
-
Saccharomyces cerevisiae 3-methylbutanol + NAD(P)+
-
r
3-pyridine carboxaldehyde + NAD(P)H highest activity Saccharomyces cerevisiae 3-pyridinemethanol + NAD(P)+
-
?
acetaldehyde + NADPH + H+
-
Saccharomyces cerevisiae ethanol + NADP+
-
r
benzaldehyde + NAD(P)H high activity Saccharomyces cerevisiae benzyl alcohol + NAD(P)+
-
?
diacetyl + NAD(P)H very low activity Saccharomyces cerevisiae ? + NAD(P)+
-
?
furaldehyde + NAD(P)H
-
Saccharomyces cerevisiae furfuryl alcohol + NAD(P)+
-
?
gluconate + NAD(P)H very low activity in both reduction and oxidation Saccharomyces cerevisiae D-glucose + NAD(P)+
-
r
glyceraldehyde + NAD(P)H
-
Saccharomyces cerevisiae glycerol + NAD(P)+
-
?
hexanal + NADPH + H+
-
Saccharomyces cerevisiae hexanol + NADP+
-
r
methyl glyoxal + NAD(P)H low activity Saccharomyces cerevisiae ? + NAD(P)+
-
?
octanal + NAD(P)H + H+
-
Saccharomyces cerevisiae octanol + NAD(P)+
-
?
pentanal + NADPH
-
Saccharomyces cerevisiae pentanol + NADP+
-
r