Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
1,5-Anhydro-D-fructose | pH 7.0, 37°C, recombinant enzyme | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, about, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADPH | Mus musculus | - |
1,5-anhydro-D-glucitol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 17.4fold from Escherichia coli strain BL21(DE3) by anion exchange and adsorption chromatography, and gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.01 | - |
purified recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADPH | - |
Mus musculus | 1,5-anhydro-D-glucitol + NADP+ | - |
? | |
1,5-anhydro-D-fructose + NADPH | preferred substrate | Mus musculus | 1,5-anhydro-D-glucitol + NADP+ | - |
? | |
2,3-butanedione + NADPH | 76.4% of the activity with 1,5-anhydro-D-fructose | Mus musculus | ? | - |
? | |
9,10-phenanthrenequinone + NADPH | 18% of the activity with 1,5-anhydro-D-fructose | Mus musculus | ? | - |
? | |
additional information | substrate specificities of the recombinant enzyme for carbonyl compounds, overview | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, about, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
AKR1E1 | - |
Mus musculus |
More | the enzyme belongs to the aldo-keto reductase family 1 | Mus musculus |
NADPH dependent 1,5-anhydro-D-fructose reductase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Mus musculus | |
NADPH | dependent on | Mus musculus |