BRENDA - Enzyme Database show
show all sequences of 1.1.1.262

Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein

Laber, B.; Maurer, W.; Scharf, S.; Stepusin, K.; Schmidt, F.S.; FEBS Lett. 449, 45-48 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(phosphohydroxy)-L-threonine + NAD+
Escherichia coli
involved in pyridoxal 5'-phosphate formation
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Escherichia coli
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, recombinant enzyme
Escherichia coli
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
pdxA gene product
Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(phosphohydroxy)-L-threonine + NAD+
involved in pyridoxal 5'-phosphate formation
207962
Escherichia coli
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
207962
Escherichia coli
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4
-
4-(phosphohydroxy)-L-threonine
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(phosphohydroxy)-L-threonine + NAD+
Escherichia coli
involved in pyridoxal 5'-phosphate formation
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Escherichia coli
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, recombinant enzyme
Escherichia coli
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
pdxA gene product
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(phosphohydroxy)-L-threonine + NAD+
involved in pyridoxal 5'-phosphate formation
207962
Escherichia coli
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH
further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
207962
Escherichia coli
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4
-
4-(phosphohydroxy)-L-threonine
-
Escherichia coli
Other publictions for EC 1.1.1.262
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654972
Banks
Biosynthesis of vitamin B6: di ...
Escherichia coli
Bioorg. Med. Chem. Lett.
14
1633-16367
2004
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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1
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656172
Sivaraman
Crystal structure of Escherich ...
Escherichia coli
J. Biol. Chem.
278
43682-43690
2003
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1
1
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1
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1
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2
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1
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3
1
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1
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1
1
1
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1
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1
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3
1
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207962
Laber
Vitamin B6 biosynthesis: forma ...
Escherichia coli
FEBS Lett.
449
45-48
1999
-
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1
-
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1
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1
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1
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1
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1
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207963
Cane
-
Biosynthesis of vitamin B6: en ...
Escherichia coli
J. Am. Chem. Soc.
121
7722-7723
1999
-
-
1
-
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1
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1
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1
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1
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1
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1
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1
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207964
Cane
-
Biosynthesis of vitamin B6: th ...
Escherichia coli
J. Am. Chem. Soc.
120
1936-1937
1998
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1
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1
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1
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1
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1
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1
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