Protein Variants | Comment | Organism |
---|---|---|
T335A | nonphosphorylatable variant | Arabidopsis thaliana |
T335D | phosphomimetic variant, exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
NADH | mutant T335D, pH 6.2, 25°C | Arabidopsis thaliana | |
0.01 | - |
NADH | mutant T335A, pH 6.2, 25°C | Arabidopsis thaliana | |
0.01 | - |
NADH | wild-type, pH 6.2, 25°C | Arabidopsis thaliana | |
0.271 | - |
NADPH | mutant T335D, pH 6.2, 25°C | Arabidopsis thaliana | |
0.353 | - |
NADPH | wild-type, pH 6.2, 25°C | Arabidopsis thaliana | |
0.408 | - |
NADPH | mutant T335A, pH 6.2, 25°C | Arabidopsis thaliana | |
9.727 | - |
glyoxylate | mutant T335D, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
15.195 | - |
glyoxylate | mutant T335A, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
16.589 | - |
glyoxylate | mutant T335A, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana | |
17.366 | - |
glyoxylate | wild-type, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
17.996 | - |
glyoxylate | wild-type, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana | |
18.011 | - |
glyoxylate | mutant T335D, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C9W5 | catalyzes reactions of EC 1.1.1.29 and EC 1.1.1.26 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADH + H+ | - |
Arabidopsis thaliana | glycolate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | reaction of EC 1.1.1.29 | Arabidopsis thaliana | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADPH + H+ | reaction of EC 1.1.1.29, NADPH is a poor cofactor for wild-type | Arabidopsis thaliana | D-glycerate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
glyoxylate | mutant T335A, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana | |
0.56 | - |
glyoxylate | wild-type, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana | |
0.56 | - |
NADPH | wild-type, pH 6.2, 25°C | Arabidopsis thaliana | |
0.62 | - |
NADPH | mutant T335A, pH 6.2, 25°C | Arabidopsis thaliana | |
0.85 | - |
NADPH | mutant T335D, pH 6.2, 25°C | Arabidopsis thaliana | |
0.92 | - |
glyoxylate | mutant T335D, cosubstrate NADPH, pH 6.2, 25°C | Arabidopsis thaliana | |
59 | - |
glyoxylate | mutant T335A, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
60 | - |
NADH | mutant T335A, pH 6.2, 25°C | Arabidopsis thaliana | |
62 | - |
NADH | wild-type, pH 6.2, 25°C | Arabidopsis thaliana | |
68 | - |
glyoxylate | wild-type, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
75 | - |
glyoxylate | mutant T335D, cosubstrate NADH, pH 6.2, 25°C | Arabidopsis thaliana | |
91 | - |
NADH | mutant T335D, pH 6.2, 25°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana | |
NADPH | NADPH is a poor cofactor for wild-type | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | HPR1 phosphomimetic variant T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity but improved NADPH-dependent activity. Complementation of the Arabidopsis HPR1 mutant by either wild-type HPR1 or HPR1 nonphosphorylatable mutant T335A fully complements the photorespiratory growth phenotype of the HPR1 mutant in ambient air, whereas HPR1 T335D-containing HPR1 mutant plants remain smaller and have lower photosynthetic CO2 assimilation rates. These phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1 T335D-complemented HPR1 mutant rosettes compared to all other HPR1-containing lines | Arabidopsis thaliana |