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Literature summary for 1.1.1.252 extracted from

  • Kristan, K.; Brunskole, M.; Stojan, J.; Rizner, T.L.
    Two homologous fungal carbonyl reductases with different substrate specificities (2009), Chem. Biol. Interact., 178, 295-302.
    View publication on PubMed
Search for more literature for 1.1.1.252

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a glutathione S-transferase (GST)-fusion protein in Escherichia coli BL21 cells Curvularia lunata

Inhibitors

Inhibitors Comment Organism Structure
3,7-dihydroxyflavone
-
 Curvularia lunata
3-hydroxyflavone
-
 Curvularia lunata
5,7-dihydroxyflavone potent inhibitor of 3HNR Curvularia lunata
5-hydroxyflavone
-
 Curvularia lunata
5-methoxyflavone
-
 Curvularia lunata
5-methyl-(1,2,4)-triazolo[3,4-b]benzothiazole tricyclazole, specific inhibitor of 3HNR Curvularia lunata
apigenin potent inhibitor of 3HNR Curvularia lunata
Biochanin A
-
 Curvularia lunata
kaempferol potent inhibitor of 3HNR Curvularia lunata
luteolin potent inhibitor of 3HNR Curvularia lunata
naringenin
-
 Curvularia lunata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,3,8-trihydroxynaphthalene + NADPH + H+ Curvularia lunata
-
 vermelone + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Curvularia lunata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity binding followed by cleavage with thrombin Curvularia lunata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3,8-trihydroxynaphthalene + NADPH + H+
-
 Curvularia lunata vermelone + NADP+
-
 ?
1,4-androstadiene-3,17-dione + NADPH + H+ poor substrate of 3HNR Curvularia lunata ? + NADP+
-
 ?
2,(5alpha)-androstene-17-one + NADPH + H+
-
 Curvularia lunata ? + NADP+
-
 ?
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one + NADP+
-
 Curvularia lunata 4,5-dihydroxy-2H-benzopyran-2-one + NADPH + H+
-
 ?
4-androstene-3,17-dione + NADPH + H+ poor substrate of 3HNR Curvularia lunata ? + NADP+
-
 ?
4-estrene-3,17-dione + NADPH + H+ poor substrate of 3HNR Curvularia lunata ? + NADP+
-
 ?
5-androstene-3beta-ol-17-one + NADPH + H+
-
 Curvularia lunata ? + NADP+
-
 ?
5alpha-androstane-3,17-dione + NADPH + H+ best steroid substrate for 3HNR Curvularia lunata ? + NADP+
-
 ?
5alpha-androstane-3-one + NADPH + H+
-
 Curvularia lunata ? + NADP+
-
 ?
5alpha-androstane-3alpha-ol-17-one + NADPH + H+
-
 Curvularia lunata ? + NADP+
-
 ?
5alpha-androstane-3beta-ol-17-one + NADPH + H+
-
 Curvularia lunata ? + NADP+
-
 ?
additional information no activity with estrone Curvularia lunata ?
-
 ?

Synonyms

Synonyms Comment Organism
3HNR
-
 Curvularia lunata
trihydroxynaphthalene reductase
-
 Curvularia lunata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 optimal temperature for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 optimal pH for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0031
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata apigenin
0.0058
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata 5,7-dihydroxyflavone
0.0068
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata luteolin
0.0079
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata kaempferol
0.012
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata Biochanin A
0.025
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata 3-hydroxyflavone
0.025
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata 5-hydroxyflavone
0.025
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata 3,7-dihydroxyflavone
0.025
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata 5-methoxyflavone
0.026
-
 at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one Curvularia lunata naringenin