Literature summary for 1.1.1.25 extracted from

Huang, K.; Li, M.; Liu, Y.; Zhu, M.; Zhao, G.; Zhou, Y.; Zhang, L.; Wu, Y.; Dai, X.; Xia, T.; Gao, L.
Functional analysis of 3-dehydroquinate dehydratase/shikimate dehydrogenases involved in shikimate pathway in Camellia sinensis (2019), Front. Plant Sci., 10, 1268 .

Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
multiple DQD/SDH protein sequences are obtained from Phytozome 12.0 and NCBI, phylogenetic analysis and tree, recombinant expression of CsDQD/SDH isozymes in Escherichia coli strain NovaBlue (DE3)	Camellia sinensis

Protein Variants

Protein Variants	Comment	Organism
G338S/G381T/D483N/L484R/D485T	site-directed mutagenesis, mutant MTCsDQD/SDHb has a similar reduction activity of 3-DHS and had six times higher oxidation activity of SA than wild-type isozyme CsDQD/SDHb, suggesting that the mutation of residues Ser338 and NRT to Gly and DI/LD in the SDH unit is the reason for the low activity of CsDQD/SDHb, respectively	Camellia sinensis
additional information	referring to the model of Arabidopsis thaliana protein crystal structure, the amino acid residue sites of Gly338, Gly381, Asp483, Leu484, and Asp485 in CsDQD/SDHb are mutated to Ser338, Thr381, Asn483, Arg484, and Thr485, respectively, the mutant protein is named MTCsDQD/SDHb	Camellia sinensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.046	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate	Camellia sinensis
0.115	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate	Camellia sinensis
0.138	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate	Camellia sinensis
0.2	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+	Camellia sinensis
0.249	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate	Camellia sinensis
0.267	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate	Camellia sinensis
0.27	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate	Camellia sinensis
0.273	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+	Camellia sinensis
0.287	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH	Camellia sinensis
0.331	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH	Camellia sinensis
0.466	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH	Camellia sinensis
0.611	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+	Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
shikimate + NADP+	Camellia sinensis	-	3-dehydroshikimate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Camellia sinensis	A0A3G3BK08	four isozymes CsDQD/SDHa-d	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant CsDQD/SDH isozymes from Escherichia coli strain NovaBlue (DE3) by maltose affinity chromatography and ultrafiltration	Camellia sinensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
bud	-	Camellia sinensis	-
leaf	first and second	Camellia sinensis	-
additional information	activities and phenolic contents in different tissues from CsDQD/SDH isozymes a-d, overview	Camellia sinensis	-
root	-	Camellia sinensis	-
stem	-	Camellia sinensis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	relative activities for 3-dehydrochikimate reduction and shikimate oxidation of purified recombinant isozymes, overview	Camellia sinensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
shikimate + NADP+	-	Camellia sinensis	3-dehydroshikimate + NADPH + H+	-	r

Synonyms

Synonyms	Comment	Organism
3-dehydroquinate dehydratase/shikimate dehydrogenase	-	Camellia sinensis
CsDQD/SDH	-	Camellia sinensis
DQD/SDH	-	Camellia sinensis
More	cf. EC 4.2.1.10	Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Camellia sinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.006	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate	Camellia sinensis
0.043	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate	Camellia sinensis
0.046	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH	Camellia sinensis
0.049	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate	Camellia sinensis
0.052	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate	Camellia sinensis
0.085	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+	Camellia sinensis
0.107	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+	Camellia sinensis
0.117	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate	Camellia sinensis
0.153	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+	Camellia sinensis
0.168	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH	Camellia sinensis
0.202	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate	Camellia sinensis
0.405	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH	Camellia sinensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Camellia sinensis

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Camellia sinensis
NADPH	-	Camellia sinensis

General Information

General Information	Comment	Organism
evolution	four CsDQD/SDH isozyme proteins are cloned from Camellia sinensis. Three CsDQD/SDH isozymes show 3-dehydroshikimate (3-DHS) reduction and shikimate (SA) oxidation functions with individual differences between the catalytic efficiency of 3-DHS reduction and SA oxidation. Isozyme CsDQD/SDHa has higher catalytic efficiency for 3-DHS reduction than for SA oxidation, isozyme CsDQD/SDHd shows the opposite tendency, and isozyme CsDQD/SDHc has almost equal catalytic efficiency for 3-DHS reduction and SA oxidation. In vitro, Gallic acid (GA) is mainly generated from 3-DHS through nonenzymatic conversion. Isozymes CsDQD/SDHc and CsDQD/ SDHd genes are involved in GA synthesis	Camellia sinensis
malfunction	the mutation of residues Ser338 and NRT to Gly and DI/LD in the SDH unit is the reason for the low activity of isozyme CsDQD/SDHb for 3-DHS reduction and SA oxidation	Camellia sinensis
metabolism	3-dehydroquinate dehydratase/shikimate dehydrogenase (DQD/SDH) is a key enzyme for catalyzing the conversion of 3-dehydroshikimate (3-DHS) to shikimate (SA). It also potentially participates in gallic acid (GA) synthesis in a branch of the SA pathway. Gallic acid (GA) is a precursor for polyphenol synthesis. The CsDQD/SDHc and CsDQD/ SDHd genes are involved in GA synthesis. In plants, DQD/SDH, a bifunctional enzyme, is crucial in the third and fourth reversible reactions in the SA pathway. Biosynthetic pathway of gallic acid and shikimic acid in plants, overview. GA could be spontaneously generated from 3-DHS in the enzymatic or nonenzymatic CsDQD/SDHs assay when using 3-DHS and the coenzyme NADP+ as substrates	Camellia sinensis
physiological function	3-dehydroquinate dehydratase/shikimate dehydrogenase (DQD/SDH) is a key enzyme for catalyzing the conversion of 3-dehydroshikimate (3-DHS) to shikimate (SA). It also potentially participates in gallic acid (GA) synthesis in a branch of the SA pathway. Quantitative RT-PCR analysis shows that CsDQD/SDHc and CsDQD/SDHd expression is correlated with GA and 1-O-galloyl-beta-D-glucose accumulation in Camellia sinensis. The SA pathway is important to plant growth, development, and defense. Polyphenols in tea plants, including phenolic acids, catechins, and flavonol derivatives, not only determine the mouthfeel of tea infusions but also provide health benefits	Camellia sinensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.099	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH	Camellia sinensis
0.13	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate	Camellia sinensis
0.139	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+	Camellia sinensis
0.195	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate	Camellia sinensis
0.197	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate	Camellia sinensis
0.374	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate	Camellia sinensis
0.508	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH	Camellia sinensis
0.535	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+	Camellia sinensis
0.56	-	shikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+	Camellia sinensis
0.748	-	NADPH	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate	Camellia sinensis
0.848	-	NADP+	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate	Camellia sinensis
1.411	-	3-dehydroshikimate	pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH	Camellia sinensis

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Release 2023.1 (January 2023)