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Literature summary for 1.1.1.25 extracted from

  • Lim, S.; Schroder, I.; Monbouquette, H.G.
    A thermostable shikimate 5-dehydrogenase from the archaeon Archaeoglobus fulgidus (2004), FEMS Microbiol. Lett., 238, 101-106.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
shikimate 87°C, pH 7.3 Archaeoglobus fulgidus
0.19
-
NADP+ 87°C, pH 7.3 Archaeoglobus fulgidus
11.4
-
NAD+ 87°C, pH 7.3 Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29140
-
2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus
32000
-
2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus
58000
-
gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
recombinant enzyme expressed in Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
732
-
87°C, pH 7.3, cosubstrate NADP+ Archaeoglobus fulgidus
1479
-
87°C, pH 7.3, cosubstrate NAD+ Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroshikimate + NADPH NADH may substitute for NADPH Archaeoglobus fulgidus shikimate + NADP+
-
r
shikimate + NAD+
-
Archaeoglobus fulgidus 3-dehydroshikimate + NADH + H+
-
r
shikimate + NADP+
-
Archaeoglobus fulgidus 3-dehydroshikimate + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE, 2 * 29140, deduced from gene sequence Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
87
-
assay at Archaeoglobus fulgidus
95
-
and above Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life about 15 days, no addition of salt Archaeoglobus fulgidus
60
-
half-life about 47 days, presence of 1 M NaCl Archaeoglobus fulgidus
87
-
half-life about 2 h, no addition of salt Archaeoglobus fulgidus
87
-
half-life about 60 h, presence of 1 M NaCl Archaeoglobus fulgidus
100
-
half-life about 2.1 h, presence of 1 M NaCl Archaeoglobus fulgidus
100
-
half-life about 30 min, no addition of salt Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
7 7.5
-
Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus