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Literature summary for 1.1.1.245 extracted from

  • Tae-Kang, K.; Choi, J.H.; Rhee, I.K.
    Purification and characterization of a cyclohexanol dehydrogenase from Rhodococcus sp. TK6 (2002), J. Microbiol. Biotechnol., 12, 39-45.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Al3+ almost complete inhibition of activity at 10 mM Rhodococcus sp.
Ca2+ inhibition of activity at 10 mM Rhodococcus sp.
Co2+ inhibition of activity at 10 mM Rhodococcus sp.
Cu2+ almost complete inhibition of activity at 10 mM Rhodococcus sp.
Mn2+ 93% inhibition of activity at 10 mM Rhodococcus sp.
Zn2+ 91% inhibition of activity at 10 mM Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
Cyclohexanol isoenzyme II at pH 7.6 Rhodococcus sp.
0.0028
-
trans-Cyclohexane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
0.0135
-
hexane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
0.0137
-
trans-cyclopentane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
0.0142
-
Cyclopentanol isoenzyme II at pH 7.6 Rhodococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ increase of activity at 10 mM Rhodococcus sp.
Mg2+ increase of activity at 10 mM Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, SDS-PAGE, isoenzyme II Rhodococcus sp.
60000
-
gel filtration, isoenzyme II Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. TK6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
9.8fold, isoenzyme II Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
127
-
purified enzyme Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. butanal + NADH
-
?
1-hexanol + NAD+ relative oxidation activity at 21% the rate of cyclohexanol oxidation Rhodococcus sp. hexanal + NADH
-
?
1-pentanol + NAD+ relative activity at 23% the rate of cyclohexanol oxidation Rhodococcus sp. n-pentanal + NADH
-
?
1-propanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. propionaldehyde + NADH
-
?
2-butanol + NAD+ relative activity at 44% the rate of cyclohexanol oxidation Rhodococcus sp. 2-butanone + NADH
-
?
2-methyl-1-butanol + NAD+ relative activity at 26% the rate of cyclohexanol oxidation Rhodococcus sp. 2-methyl-1-butanal + NADH
-
?
2-methyl-1-propanol + NAD+ relative activity at 17% the rate of cyclohexanol oxidation Rhodococcus sp. isobutyraldehyde + NADH
-
?
2-methyl-2-propanol + NAD+ relative activity at 16% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
?
2-propanol + NAD+ relative activity at 17% the rate of cyclohexanol oxidation Rhodococcus sp. acetone + NADH
-
?
cis-cyclopentane-1,2-diol + NAD+ relative activity at 27% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 cyclohexane-1,2-dione + NADH
-
?
cyclohexane-1,3-diol + NAD+ relative oxidation activity at 28% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,3-dione + NADH
-
?
cyclohexane-1,4-diol + NAD+ relative oxidation activity at 22% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,4-dione + NADH
-
?
cyclohexanol + NAD+
-
Rhodococcus sp. cyclohexanone + NADH
-
?
cyclohexanol + NAD+
-
Rhodococcus sp. TK6 cyclohexanone + NADH
-
?
cyclopentanol + NAD+ relative oxidation activity at 77% the rate of cyclohexanol oxidation Rhodococcus sp. cyclopentanone + NADH
-
?
cyclopentanol + NAD+ relative oxidation activity at 77% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 cyclopentanone + NADH
-
?
ethane-1,2-diol + NAD+ relative activity at 5% the rate of cyclohexanol oxidation Rhodococcus sp. glyoxal + NADH
-
?
ethanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. acetaldehyde + NADH
-
?
ethanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 acetaldehyde + NADH
-
?
hexane-1,2-diol + NAD+ relative activity at 84% the rate of cyclohexanol oxidation Rhodococcus sp. 2-oxohexanal + NADH
-
?
hexane-1,6-diol + NAD+ relative activity at 21% the rate of cyclohexanol oxidation Rhodococcus sp. hexanedial + NADH
-
?
methanol + NAD+ relative activity at 2% the rate of cyclohexanol oxidation Rhodococcus sp. formaldehyde + NADH + H+
-
?
methanol + NAD+ relative activity at 2% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 formaldehyde + NADH + H+
-
?
propane-1,2-diol + NAD+ relative activity at 30% the rate of cyclohexanol oxidation Rhodococcus sp. methylglyoxal + NADH
-
?
propane-1,3-diol + NAD+ relative activity at 7% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
?
trans-cyclohexane-1,2-diol + NAD+
-
Rhodococcus sp. cyclohexane-1,2-dione + NADH + H+
-
?
trans-cyclopentane-1,2-diol + NAD+ relative activity at 114% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE, isoenzyme II Rhodococcus sp.

Synonyms

Synonyms Comment Organism
cyclohexanol dehydrogenase II isoenzyme Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
labile Rhodococcus sp.
40
-
90% activity after incubation for 20 min Rhodococcus sp.
50
-
less than 20% activity after incubation for 20 min Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 stable Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ isoenzyme II Rhodococcus sp.