Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 100% inhibition | Nocardia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NAD+ | - |
Nocardia sp. | |
0.037 | - |
Cyclohexanol | - |
Nocardia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
gel filtration | Nocardia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
126fold, affinity chromatograpy | Nocardia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
143.5 | - |
purified enzyme | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | poor substrate | Nocardia sp. | butanal + NADH | - |
? | |
1-decanol + NAD+ | poor substrate | Nocardia sp. | dodecanal + NADH | - |
? | |
1-dodecanol + NAD+ | poor substrate | Nocardia sp. | dodecanal + NADH | - |
? | |
1-heptanol + NAD+ | poor substrate | Nocardia sp. | heptanal + NADH | - |
? | |
1-octanol + NAD+ | poor substrate | Nocardia sp. | octanal + NADH | - |
? | |
2,3-butanediol + NAD+ | relative activity at 4% the rate of cyclohexanol oxidation | Nocardia sp. | ? + NADH | - |
? | |
2,3-dimethylcyclohexanol + NAD+ | relative activity at 78% the rate of cyclohexanol oxidation | Nocardia sp. | 2,3-dimethylcyclohexanone + NADH | - |
? | |
2,6-dimethylcyclohexanol + NAD+ | relative activity at 14% the rate of cyclohexanol oxidation | Nocardia sp. | 2,6-dimethylcyclohexanone + NADH | - |
? | |
2-methyl-1-pentanol + NAD+ | poor substrate | Nocardia sp. | 2-methylpentanal + NADH | - |
? | |
2-methylcyclohexanol + NAD+ | relative activity at 51% the rate of cyclohexanol oxidation | Nocardia sp. | 2-methylcyclohexanone + NADH | - |
? | |
2-pentanol + NAD+ | relative activity at 43% the rate of cyclohexanol oxidation | Nocardia sp. | 2-pentanone + NADH | - |
? | |
2-propanol + NAD+ | relative activity at 19% the rate of cyclohexanol oxidation | Nocardia sp. | acetone + NADH | - |
? | |
3-cyclohexyl-1-propanol + NAD+ | poor substrate | Nocardia sp. | 3-cyclohexyl-1-propanal + NADH | - |
? | |
3-hexanol + NAD+ | relative activity at 196% the rate of cyclohexanol oxidation | Nocardia sp. | 3-hexanone + NADH | - |
? | |
3-methylcyclohexanol + NAD+ | relative activity at 74% the rate of cyclohexanol oxidation | Nocardia sp. | 3-methylcyclohexanone + NADH | - |
? | |
4-heptanol + NAD+ | relative activity at 125% the rate of cyclohexanol oxidation | Nocardia sp. | 4-heptanone + NADH | - |
? | |
4-methyl-1-pentanol + NAD+ | poor substrate | Nocardia sp. | 4-methylpentanal + NADH | - |
? | |
4-methylcyclohexanol + NAD+ | relative activity at 66% the rate of cyclohexanol oxidation | Nocardia sp. | 4-methylcyclohexanone + NADH | - |
? | |
benzyl alcohol + NAD+ | poor substrate | Nocardia sp. | benzaldehyde + NADH | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity at 43% the rate of cyclohexanol oxidation | Nocardia sp. | cyclohexane-1,2-dione + NADH | - |
? | |
cyclohexane-1,3-diol + NAD+ | relative oxidation activity at 96% the rate of cyclohexanol oxidation | Nocardia sp. | cyclohexane-1,3-dione + NADH | - |
? | |
cyclohexane-1,4-diol + NAD+ | relative oxidation activity at 91% the rate of cyclohexanol oxidation | Nocardia sp. | cyclohexane-1,4-dione + NADH | - |
? | |
cyclohexanol + NAD+ | - |
Nocardia sp. | cyclohexanone + NADH | reverse reaction at pH 7 | ? | |
cyclooctanol + NAD+ | relative activity at 500% the rate of cyclohexanol oxidation | Nocardia sp. | cyclooctanone + NADH | - |
? | |
cyclopentanol + NAD+ | relative oxidation activity at 232% the rate of cyclohexanol oxidation | Nocardia sp. | cyclopentanone + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 min 50% loss of activity | Nocardia sp. |
55 | - |
1 min 85% loss of activity | Nocardia sp. |
60 | - |
1 min 95% loss of activity | Nocardia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Nocardia sp. |