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Literature summary for 1.1.1.245 extracted from

  • Stirling, L.A.; Perry, J.J.
    Purification and properties of a nicotinamide adenine dinucleotide-linked cyclohexanol dehydrogenase from a Nocardia species (1980), Curr. Microbiol., 4, 37-40.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 100% inhibition Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NAD+
-
Nocardia sp.
0.037
-
Cyclohexanol
-
Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
gel filtration Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
126fold, affinity chromatograpy Nocardia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
143.5
-
purified enzyme Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+ poor substrate Nocardia sp. butanal + NADH
-
?
1-decanol + NAD+ poor substrate Nocardia sp. dodecanal + NADH
-
?
1-dodecanol + NAD+ poor substrate Nocardia sp. dodecanal + NADH
-
?
1-heptanol + NAD+ poor substrate Nocardia sp. heptanal + NADH
-
?
1-octanol + NAD+ poor substrate Nocardia sp. octanal + NADH
-
?
2,3-butanediol + NAD+ relative activity at 4% the rate of cyclohexanol oxidation Nocardia sp. ? + NADH
-
?
2,3-dimethylcyclohexanol + NAD+ relative activity at 78% the rate of cyclohexanol oxidation Nocardia sp. 2,3-dimethylcyclohexanone + NADH
-
?
2,6-dimethylcyclohexanol + NAD+ relative activity at 14% the rate of cyclohexanol oxidation Nocardia sp. 2,6-dimethylcyclohexanone + NADH
-
?
2-methyl-1-pentanol + NAD+ poor substrate Nocardia sp. 2-methylpentanal + NADH
-
?
2-methylcyclohexanol + NAD+ relative activity at 51% the rate of cyclohexanol oxidation Nocardia sp. 2-methylcyclohexanone + NADH
-
?
2-pentanol + NAD+ relative activity at 43% the rate of cyclohexanol oxidation Nocardia sp. 2-pentanone + NADH
-
?
2-propanol + NAD+ relative activity at 19% the rate of cyclohexanol oxidation Nocardia sp. acetone + NADH
-
?
3-cyclohexyl-1-propanol + NAD+ poor substrate Nocardia sp. 3-cyclohexyl-1-propanal + NADH
-
?
3-hexanol + NAD+ relative activity at 196% the rate of cyclohexanol oxidation Nocardia sp. 3-hexanone + NADH
-
?
3-methylcyclohexanol + NAD+ relative activity at 74% the rate of cyclohexanol oxidation Nocardia sp. 3-methylcyclohexanone + NADH
-
?
4-heptanol + NAD+ relative activity at 125% the rate of cyclohexanol oxidation Nocardia sp. 4-heptanone + NADH
-
?
4-methyl-1-pentanol + NAD+ poor substrate Nocardia sp. 4-methylpentanal + NADH
-
?
4-methylcyclohexanol + NAD+ relative activity at 66% the rate of cyclohexanol oxidation Nocardia sp. 4-methylcyclohexanone + NADH
-
?
benzyl alcohol + NAD+ poor substrate Nocardia sp. benzaldehyde + NADH
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity at 43% the rate of cyclohexanol oxidation Nocardia sp. cyclohexane-1,2-dione + NADH
-
?
cyclohexane-1,3-diol + NAD+ relative oxidation activity at 96% the rate of cyclohexanol oxidation Nocardia sp. cyclohexane-1,3-dione + NADH
-
?
cyclohexane-1,4-diol + NAD+ relative oxidation activity at 91% the rate of cyclohexanol oxidation Nocardia sp. cyclohexane-1,4-dione + NADH
-
?
cyclohexanol + NAD+
-
Nocardia sp. cyclohexanone + NADH reverse reaction at pH 7 ?
cyclooctanol + NAD+ relative activity at 500% the rate of cyclohexanol oxidation Nocardia sp. cyclooctanone + NADH
-
?
cyclopentanol + NAD+ relative oxidation activity at 232% the rate of cyclohexanol oxidation Nocardia sp. cyclopentanone + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
1 min 50% loss of activity Nocardia sp.
55
-
1 min 85% loss of activity Nocardia sp.
60
-
1 min 95% loss of activity Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Nocardia sp.