KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NAD+ | - |
Pseudomonas sp. | |
0.05 | - |
Cyclohexanol | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanol + NAD+ | Pseudomonas sp. | inducible | cyclohexanone + NADH | - |
? | |
cyclohexanol + NAD+ | Pseudomonas sp. | metabolism of cyclohexane | cyclohexanone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
oxidation of cyclohexanol at pH 7.8 | Pseudomonas sp. |
1.9 | - |
reduction of cyclohexanone at pH 7.8 | Pseudomonas sp. |
2.5 | - |
pH 10 | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for several days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-cycloheptanediol + NAD+ | relative oxidation activity at 25% the rate of cyclohexanol oxidation | Pseudomonas sp. | cycloheptane-1,2-dione + NADH | - |
? | |
1-heptanol + NAD+ | relative oxidation activity at 32% the rate of cyclohexanol oxidation | Pseudomonas sp. | heptanal + NADH | - |
? | |
1-hexanol + NAD+ | relative oxidation activity at 7% the rate of cyclohexanol oxidation | Pseudomonas sp. | hexanal + NADH | - |
? | |
2-cyclohexanol + NAD+ | relative oxidation activity at 142% the rate of cyclohexanol oxidation | Pseudomonas sp. | 2-cyclohexenone + NADH | slow reduction | r | |
2-hydroxycyclohexanone + NADH | relative activity at 50% the rate of cyclohexanone reduction | Pseudomonas sp. | ? + NAD+ | - |
? | |
cycloheptanone + NADH | relative activity at 10% the rate of cyclohexanone reduction | Pseudomonas sp. | cycloheptanol + NAD+ | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity at 15% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,2-dione + NADH | slow reduction | r | |
cyclohexane-1,3-diol + NAD+ | relative oxidation activity at 14% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,3-dione + NADH | slow reduction | r | |
cyclohexane-1,4-diol + NAD+ | relative oxidation activity at 36% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,4-dione + NADH | relative reduction activity at 240% the rate of cyclohexanone reduction | r | |
cyclohexanol + NAD+ | - |
Pseudomonas sp. | cyclohexanone + NADH | - |
r | |
cyclohexanol + NAD+ | inducible | Pseudomonas sp. | cyclohexanone + NADH | - |
? | |
cyclohexanol + NAD+ | metabolism of cyclohexane | Pseudomonas sp. | cyclohexanone + NADH | - |
? | |
cyclopentanol + NAD+ | - |
Pseudomonas sp. | cyclopentanone + NADH | relative reduction activity at 4% the rate of cyclohexanone reduction | r | |
additional information | 2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+ | Pseudomonas sp. | additional information | 2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
rapid inactivation above pH 10.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. | |
NADP+ | less than 5% activity than with NAD+ | Pseudomonas sp. |