Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Act | activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus coagulans Adh is increased by the endogenous activator protein Act, a Nudix hydrolase | Weizmannia coagulans | |
Act | activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, no activation in vivo | Bacillus methanolicus | |
Act | activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh3 is increased by the endogenous activator protein Act, a Nudix hydrolase | Bacillus methanolicus | |
Act | activator protein, UniProt ID I3EA59, the in vitro activity of Desulfitobacterium hafniense Adh is increased by the endogenous activator protein Act, a Nudix hydrolase | Desulfitobacterium hafniense | |
Act protein | activator protein, UniProt ID I3EA59, the in vitro activity of Lysinibacillus sphaericus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase | Lysinibacillus sphaericus | |
additional information | no activation by activator protein Act, UniProt ID I3EA59 | Lysinibacillus fusiformis |
Cloned (Comment) | Organism |
---|---|
gene BFZC1_05383, recombinant expression in Escherichia coli | Lysinibacillus fusiformis |
gene Bsph_4187, recombinant expression in Escherichia coli | Lysinibacillus sphaericus |
gene mdh, recombinant expression in Escherichia coli | Bacillus methanolicus |
gene mdh1, recombinant expression in Escherichia coli | Bacillus methanolicus |
gene mdh2, recombinant expression in Escherichia coli | Bacillus methanolicus |
gene mdh3, recombinant expression in Escherichia coli | Bacillus methanolicus |
recombinant enzyme expression in Escherichia coli | Weizmannia coagulans |
recombinant enzyme expression in Escherichia coli | Desulfitobacterium hafniense |
recombinant enzyme expression in Escherichia coli | Desulfofundulus kuznetsovii |
Protein Variants | Comment | Organism |
---|---|---|
D38G | site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme | Bacillus methanolicus |
D41G | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Bacillus methanolicus |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Weizmannia coagulans |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Desulfitobacterium hafniense |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Lysinibacillus fusiformis |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Bacillus methanolicus |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Lysinibacillus sphaericus |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview | Desulfofundulus kuznetsovii |
additional information | utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), up to 40% incorporation of methanol into central metabolites is achieved, system evaluation, overview | Bacillus methanolicus |
S97G | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Bacillus methanolicus |
S97G | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Weizmannia coagulans | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Desulfitobacterium hafniense | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Lysinibacillus fusiformis | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Bacillus methanolicus | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Lysinibacillus sphaericus | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Desulfofundulus kuznetsovii | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Desulfofundulus kuznetsovii MGA3 | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Lysinibacillus fusiformis ZC1 | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Desulfitobacterium hafniense Y51 | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Bacillus methanolicus PB1 | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Weizmannia coagulans 36D1 | - |
formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | Bacillus methanolicus MGA3 | - |
formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | I3DTM5 | - |
- |
Bacillus methanolicus | I3DTP7 | - |
- |
Bacillus methanolicus | I3DVX6 | - |
- |
Bacillus methanolicus | I3DX19 | - |
- |
Bacillus methanolicus | I3E2P9 | - |
- |
Bacillus methanolicus | I3E949 | - |
- |
Bacillus methanolicus MGA3 | I3DTM5 | - |
- |
Bacillus methanolicus MGA3 | I3E2P9 | - |
- |
Bacillus methanolicus MGA3 | I3E949 | - |
- |
Bacillus methanolicus PB1 | I3DTP7 | - |
- |
Bacillus methanolicus PB1 | I3DVX6 | - |
- |
Bacillus methanolicus PB1 | I3DX19 | - |
- |
Desulfitobacterium hafniense | - |
- |
- |
Desulfitobacterium hafniense Y51 | - |
- |
- |
Desulfofundulus kuznetsovii | - |
- |
- |
Desulfofundulus kuznetsovii MGA3 | - |
- |
- |
Lysinibacillus fusiformis | - |
- |
- |
Lysinibacillus fusiformis ZC1 | - |
- |
- |
Lysinibacillus sphaericus | B1HX72 | - |
- |
Weizmannia coagulans | - |
- |
- |
Weizmannia coagulans 36D1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity of the recombinant enzyme in Escherichia coli suspension cells | Lysinibacillus fusiformis |
additional information | - |
no activity of the recombinant enzyme in Escherichia coli suspension cells | Bacillus methanolicus |
0.0007 | - |
recombinant wild-type Mdh in Escherichia coli suspension cells, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.0007 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.001 | - |
recombinant wild-type Adh in Escherichia coli suspension cells, pH 7.4, 37°C | Lysinibacillus sphaericus |
0.0014 | - |
recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0015 | - |
recombinant wild-type Mdh3 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0017 | - |
recombinant wild-type Mdh in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.0018 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Desulfitobacterium hafniense |
0.0023 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0026 | - |
recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0029 | - |
recombinant wild-type Adh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Lysinibacillus sphaericus |
0.003 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Weizmannia coagulans |
0.003 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.0033 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0038 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Lysinibacillus fusiformis |
0.0038 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Lysinibacillus fusiformis |
0.0051 | - |
recombinant mutant S97G Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0057 | - |
recombinant mutant S97G Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0058 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Weizmannia coagulans |
0.007 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Desulfitobacterium hafniense |
0.008 | 4 | recombinant wild-type Adh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Lysinibacillus sphaericus |
0.01 | - |
recombinant wild-type Mdh1 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C | Bacillus methanolicus |
0.0114 | - |
recombinant mutant S97G Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.014 | - |
recombinant mutant S97G Mdh in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.0155 | - |
recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.0155 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Weizmannia coagulans |
0.0167 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Desulfitobacterium hafniense |
0.021 | - |
recombinant mutant S97G Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.0213 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.023 | - |
recombinant wild-type Mdh3 in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.0273 | - |
recombinant wild-type Mdh3 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.03 | - |
recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C | Bacillus methanolicus |
0.045 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act | Bacillus methanolicus |
0.22 | - |
recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 9.5, 45°C | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | - |
Weizmannia coagulans | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Desulfitobacterium hafniense | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Lysinibacillus fusiformis | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Lysinibacillus sphaericus | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Desulfofundulus kuznetsovii | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Desulfofundulus kuznetsovii MGA3 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Lysinibacillus fusiformis ZC1 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Desulfitobacterium hafniense Y51 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Bacillus methanolicus PB1 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Weizmannia coagulans 36D1 | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Bacillus methanolicus MGA3 | formaldehyde + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Weizmannia coagulans |
ADH | - |
Desulfitobacterium hafniense |
ADH | - |
Lysinibacillus fusiformis |
ADH | - |
Lysinibacillus sphaericus |
ADH | - |
Desulfofundulus kuznetsovii |
BFZC1_05383 | - |
Lysinibacillus fusiformis |
Bsph_4187 | - |
Lysinibacillus sphaericus |
MDH | - |
Bacillus methanolicus |
Mdh1 | - |
Bacillus methanolicus |
MDH2 | - |
Bacillus methanolicus |
Mdh3 | - |
Bacillus methanolicus |
NAD-dependent methanol dehydrogenase | - |
Weizmannia coagulans |
NAD-dependent methanol dehydrogenase | - |
Desulfitobacterium hafniense |
NAD-dependent methanol dehydrogenase | - |
Lysinibacillus fusiformis |
NAD-dependent methanol dehydrogenase | - |
Bacillus methanolicus |
NAD-dependent methanol dehydrogenase | - |
Lysinibacillus sphaericus |
NAD-dependent methanol dehydrogenase | - |
Desulfofundulus kuznetsovii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Weizmannia coagulans |
37 | - |
assay at | Desulfitobacterium hafniense |
37 | - |
assay at | Lysinibacillus fusiformis |
37 | - |
assay at | Bacillus methanolicus |
37 | - |
assay at | Lysinibacillus sphaericus |
37 | - |
assay at | Desulfofundulus kuznetsovii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Weizmannia coagulans |
7.4 | - |
assay at | Desulfitobacterium hafniense |
7.4 | - |
assay at | Lysinibacillus fusiformis |
7.4 | - |
assay at | Bacillus methanolicus |
7.4 | - |
assay at | Lysinibacillus sphaericus |
7.4 | - |
assay at | Desulfofundulus kuznetsovii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Weizmannia coagulans | |
NAD+ | - |
Desulfitobacterium hafniense | |
NAD+ | - |
Lysinibacillus fusiformis | |
NAD+ | - |
Bacillus methanolicus | |
NAD+ | - |
Lysinibacillus sphaericus | |
NAD+ | - |
Desulfofundulus kuznetsovii |