Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | strongly stimulated by the ACT protein | Bacillus methanolicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha | Bacillus methanolicus |
Protein Variants | Comment | Organism |
---|---|---|
D100N | strongly reduced NAD+-binding, no activity | Bacillus methanolicus |
D88N | only minor effects on activity | Bacillus methanolicus |
G13A | only minor effects on activity | Bacillus methanolicus |
G15A | only minor effects on activity | Bacillus methanolicus |
G95A | impaired cofactor binding, low acitivity | Bacillus methanolicus |
K103R | strongly reduced NAD+-binding, no activity | Bacillus methanolicus |
S97G | impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein | Bacillus methanolicus |
S97T | impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein | Bacillus methanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | native enzyme, pH 9.5, 50°C | Bacillus methanolicus | |
0.04 | - |
NAD+ | recombinant enzyme without added Mg2+, pH 9.5, 50°C | Bacillus methanolicus | |
0.2 | - |
NAD+ | S97T mutant enzyme without added Mg2+, pH 9.5, 50°C | Bacillus methanolicus | |
2.5 | - |
NAD+ | S97G mutant enzyme without added Mg2+, pH 9.5, 50°C | Bacillus methanolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | bound to enzyme, essential for binding of NAD+ | Bacillus methanolicus | |
Zn2+ | bound to enzyme | Bacillus methanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
10 * 43000, SDS-PAGE | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Bacillus methanolicus | - |
formaldehyde + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzymes | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 43000, SDS-PAGE | Bacillus methanolicus |
Synonyms | Comment | Organism |
---|---|---|
More | not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8 | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | tightly but not covalentely bound to enzyme, a second NAD+ is required for the regeneration of bound NADH | Bacillus methanolicus |