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Literature summary for 1.1.1.239 extracted from

  • Payne, D.W.; Talalay, P.
    Isolation of novel microbiol 3alpha-, 3beta, and 17beta-hydroxysteroid dehydrogenases (1985), J. Biol. Chem., 260, 13648-13655.
    View publication on PubMed
Search for more literature for 1.1.1.239

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 androst-4-ene-3,17-dione
-
 Alcaligenes sp.
0.0036
-
 testosterone
-
 Alcaligenes sp.
0.0064
-
 17beta-estradiol
-
 Alcaligenes sp.
0.009
-
 testosterone cofactor S-NAD+ Alcaligenes sp.
0.0139
-
 17beta-estradiol cofactor S-NAD+ Alcaligenes sp.
0.027
-
 NAD+
-
 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Alcaligenes sp.
68000
-
 gel filtration Alcaligenes sp.
86000
-
 native PAGE Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + NAD+ Alcaligenes sp.
-
 androst-4-ene-3,17-dione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE cellulose, phenyl-Sepharose, Red-agarose Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
335
-
-
 Alcaligenes sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM potassium phosphate, 50% glycerol, 1 mM dithiotreitol, 1 mM EDTA, pH 7.5, 1 year, no loss in activity Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11alpha-hydroxytestosterone + NAD+ 156% velocity compared to testosterone Alcaligenes sp. 11alpha-hydroxyandrost-4-ene-3,17-dione + NADH
-
 r
11beta-hydroxytestosterone + NAD+ 136% velocity compared to testosterone Alcaligenes sp. 11beta-hydroxyandrost-4-ene-3,17-dione + NADH
-
 r
15alpha-hydroxytestosterone + NAD+ 131% velocity compared to testosterone Alcaligenes sp. 15alpha-hydroxyandrost-4-ene-3,17-dione + NADH
-
 r
17beta-hydroxy-5beta-androstan-3-one + NAD+ 86% velocity compared to testosterone Alcaligenes sp. 5beta-androstane-3,17-dione + NADH
-
 r
5alpha-androstane-3alpha,17beta-diol + NAD+ 72% velocity compared to testosterone Alcaligenes sp. 5alpha-androstane-3alpha-ol-17-one + NADH
-
 r
5alpha-androstane-3beta,17beta-diol + NAD+ 72% velocity compared to testosterone Alcaligenes sp. 5alpha-androstane-3beta-ol-17-one + NADH
-
 r
5alpha-dihydrotestosterone + NAD+ 133% velocity compared to testosterone Alcaligenes sp. 5alpha-androstane-3,17-dione + NADH + H+
-
 r
5beta-androstane-3alpha,17beta-diol + NAD+ 57% velocity compared to testosterone Alcaligenes sp. 5beta-androstane-3alpha-ol-17-one + NADH
-
 r
6beta-hydroxytestosterone + NAD+ 84% velocity compared to testosterone Alcaligenes sp. 6beta-hydroxyandrost-4-ene-3,17-dione + NADH
-
 r
androst-4-ene-3beta,17beta-diol + NAD+ 75% velocity compared to testosterone Alcaligenes sp. androst-4-ene-3beta-ol-17-one + NADH
-
 r
androst-5-ene-3beta,17beta-diol + NAD+ 33% velocity compared to testosterone Alcaligenes sp. androst-5-ene-3beta-ol-17-one + NADH
-
 r
estradiol + NAD+ 72% velocity compared to testosterone oxidation Alcaligenes sp. estrone + NADH
-
 r
testosterone + NAD+
-
 Alcaligenes sp. androst-4-ene-3,17-dione + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20% activity lost after 1 h in the presence of 52.5% glycerol, half-time for heat inactivation 120 min, activity is rapidly lost above 50°C Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of androst-4-ene-3,17-dione Alcaligenes sp.
9 10 oxidation of testosterone, equal velocity of oxidation and reduction at pH 8.0 Alcaligenes sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Alcaligenes sp.