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Literature summary for 1.1.1.239 extracted from
- Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver (1990), Biochem. J., 266, 583-589.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 21-hydroxypregn-4-ene-3,20-dione | 0.010 mM, 73% inhibition | Mesocricetus auratus |  |
| 5alpha-Androstan-3beta-ol-17-one | 0.005 mM, 55% inhibition | Mesocricetus auratus | |
| 5alpha-pregnan-3beta-ol-20-one | 0.005 mM, 52% inhibition | Mesocricetus auratus | |
| 5beta-androstan-3beta-ol-17-one | 0.005 mM, 87% inhibition | Mesocricetus auratus | |
| 5beta-Pregnan-3beta-ol-20-one | 0.005 mM, 81% inhibition | Mesocricetus auratus | |
| dexamethasone | 0.10 mM, 39% inhibition | Mesocricetus auratus | |
| Dienstrol | 0.010 mM, 60% inhibition | Mesocricetus auratus | |
| Hexoestrol | 0.010 mM, 62% inhibition | Mesocricetus auratus | |
| indomethacin | 0.10 mM, 67% inhibition | Mesocricetus auratus | |
| Medroxyprogesterone acetate | 0.010 mM, 22% inhibition | Mesocricetus auratus | |
| stilboestrol | 0.010 mM, 61% inhibition | Mesocricetus auratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
lithocholic acid | - |
Mesocricetus auratus | |
| 0.0022 | - |
testosterone | - |
Mesocricetus auratus | |
| 0.003 | - |
glycolithocholic acid | - |
Mesocricetus auratus | |
| 0.0031 | - |
5alpha-androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
| 0.0038 | - |
5beta-pregnan-3,20-dione | - |
Mesocricetus auratus | |
| 0.0046 | - |
5beta-Pregnan-3alpha-ol-20-one | - |
Mesocricetus auratus | |
| 0.0048 | - |
5beta-Androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
| 0.0049 | - |
5beta-androstan-17beta-ol-3-one | - |
Mesocricetus auratus | |
| 0.0062 | - |
5beta-pregnan-3alpha,17beta-diol-20-one | - |
Mesocricetus auratus | |
| 0.0066 | - |
5beta-androstan-3,17-dione | - |
Mesocricetus auratus | |
| 0.0075 | - |
glycochenodeoxycholic acid | - |
Mesocricetus auratus | |
| 0.02 | - |
NADH | - |
Mesocricetus auratus | |
| 0.27 | - |
NAD+ | - |
Mesocricetus auratus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mesocricetus auratus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Mesocricetus auratus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM Tris/HCl buffer, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA | Mesocricetus auratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-androstan-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3alpha-ol-17-one + NADH | - |
? | |
| 5alpha-pregnan-3,21alpha-diol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-21-ol-3,20-dione + NADH | - |
? | |
| 5beta-androstan-17beta-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH | - |
? | |
| 5beta-androstan-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3alpha-ol-17-one + NADH | - |
? | |
| 5beta-pregnan-3alpha,21-diol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-21-ol-3,20-dione + NADH | - |
? | |
| 5beta-pregnan-3alpha-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH | - |
? | |
| glycochenodeoxycholic acid + NAD+ | - |
Mesocricetus auratus | 7alpha-hydroxy-3-oxo-5-beta-cholanoyl glycine + NADH | - |
? | |
| glycolithocholic acid + NAD+ | - |
Mesocricetus auratus | 3-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
| lithocholic acid + NAD+ | - |
Mesocricetus auratus | 5beta-cholan-3-one-24-oic acid + NADH | - |
? | |
| testosterone + NAD+ | - |
Mesocricetus auratus | androst-4-ene-3,17-dione + NADH + H+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life 2.3 min | Mesocricetus auratus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
glycochenodeoxycholic acid | - |
Mesocricetus auratus | |
| 0.05 | - |
lithocholic acid | - |
Mesocricetus auratus | |
| 0.158 | - |
5beta-pregnan-3,20-dione | - |
Mesocricetus auratus | |
| 0.167 | - |
5beta-androstan-3,17-dione | - |
Mesocricetus auratus | |
| 0.233 | - |
5beta-Pregnan-3alpha-ol-20-one | - |
Mesocricetus auratus | |
| 0.25 | - |
glycolithocholic acid | - |
Mesocricetus auratus | |
| 0.267 | - |
5alpha-Pregnan-3alpha,21-diol-20-one | - |
Mesocricetus auratus | |
| 0.367 | - |
5beta-androstan-17beta-ol-3-one | - |
Mesocricetus auratus | |
| 0.367 | - |
5beta-Pregnan-3alpha,21-diol-20-one | - |
Mesocricetus auratus | |
| 0.417 | - |
testosterone | - |
Mesocricetus auratus | |
| 0.45 | - |
5alpha-androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
| 0.85 | - |
5beta-Androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
oxidation of 5beta-androstan-3alpha,17beta-diol | Mesocricetus auratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | preferred | Mesocricetus auratus | |
| NADH | preferred | Mesocricetus auratus | |
| NADP+ | 15% of NAD+-activity | Mesocricetus auratus | |
| NADPH | - |
Mesocricetus auratus | |
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