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Literature summary for 1.1.1.236 extracted from
- Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism (2006), Phytochemistry, 67, 327-337.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Solanum tuberosum |
| expressed in Escherichia coli | Datura stramonium |
| expressed in Escherichia coli | Atropa belladonna |
| expressed in Escherichia coli | Hyoscyamus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NADPH | - |
Solanum tuberosum |  |
| 0.0021 | - |
NADPH | - |
Atropa belladonna | |
| 0.0061 | - |
NADPH | - |
Hyoscyamus niger | |
| 0.016 | - |
NADPH | at pH 5.95 | Datura stramonium | |
| 0.033 | - |
tropinone | at pH 6.4 | Solanum tuberosum | |
| 0.033 | - |
tropinone | at pH 5.95 | Datura stramonium | |
| 0.034 | - |
tropinone | at pH 5.9 | Hyoscyamus niger | |
| 0.09 | - |
tropinone | at pH 6.25 | Atropa belladonna | |
| 0.265 | - |
N-propyl-4-piperidinone | - |
Hyoscyamus niger | |
| 0.38 | - |
4-tetrahydro-thiopyranone | - |
Atropa belladonna | |
| 0.534 | - |
4-ethyl-cyclohexanone | - |
Hyoscyamus niger | |
| 0.65 | - |
N-methyl-4-piperidinone | - |
Atropa belladonna | |
| 0.77 | - |
N-methyl-4-piperidinone | - |
Hyoscyamus niger | |
| 2 | - |
4-tetrahydro-thiopyranone | - |
Datura stramonium | |
| 2.03 | - |
4-methyl-cyclohexanone | - |
Hyoscyamus niger | |
| 2.8 | - |
4-methyl-cyclohexanone | - |
Datura stramonium | |
| 7.58 | - |
3-methyl-cyclohexanone | - |
Hyoscyamus niger | |
| 20 | - |
N-methyl-4-piperidinone | - |
Datura stramonium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28310 | - |
calculation from sequence of cDNA | Solanum tuberosum |
| 28310 | - |
calculation from sequence of cDNA | Datura stramonium |
| 28310 | - |
calculation from sequence of cDNA | Atropa belladonna |
| 28310 | - |
calculation from sequence of cDNA | Hyoscyamus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Atropa belladonna | - |
- |
- |
| Datura stramonium | - |
- |
- |
| Hyoscyamus niger | - |
- |
- |
| Solanum tuberosum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Solanum tuberosum | - |
| root | - |
Datura stramonium | - |
| root | - |
Atropa belladonna | - |
| root | - |
Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Solanum tuberosum |
| additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Datura stramonium |
| additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Atropa belladonna |
| additional information | - |
quinuclidinone and 8-thiabicylo[3.2.1]octan-3-one are no substrates of TRII | Hyoscyamus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 4-ethyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| 4-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 4-tetrahydro-thiopyranone + NADP+ | - |
Datura stramonium | ? | - |
? | |
| 4-tetrahydro-thiopyranone + NADPH | - |
Atropa belladonna | ? + NADP+ | - |
? | |
| N-methyl-4-piperidinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| N-methyl-4-piperidinone + NADPH | - |
Atropa belladonna | ? + NADP+ | - |
? | |
| N-methyl-4-piperidinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| N-propyl-4-piperidinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| tropinone + NADPH + H+ | - |
Solanum tuberosum | pseudotropine + NADP+ | - |
ir | |
| tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
ir | |
| tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
ir | |
| tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pseudotropine forming tropinone reductase | - |
Solanum tuberosum |
| pseudotropine forming tropinone reductase | - |
Datura stramonium |
| pseudotropine forming tropinone reductase | - |
Atropa belladonna |
| pseudotropine forming tropinone reductase | - |
Hyoscyamus niger |
| TRII | - |
Solanum tuberosum |
| TRII | - |
Datura stramonium |
| TRII | - |
Atropa belladonna |
| TRII | - |
Hyoscyamus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.73 | - |
tropinone | at pH 7.0 | Datura stramonium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Solanum tuberosum |
| 5.8 | - |
- |
Hyoscyamus niger |
| 6.25 | - |
- |
Datura stramonium |
| 6.25 | - |
- |
Atropa belladonna |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Solanum tuberosum | |
| NADPH | - |
Datura stramonium | |
| NADPH | - |
Atropa belladonna | |
| NADPH | - |
Hyoscyamus niger | |
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Release 2023.1 (January 2023)
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