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Literature summary for 1.1.1.236 extracted from
- The reduction of tropinone in Datura stramonium root cultures by two specific reductases (1994), Phytochemistry, 37, 391-400.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Datura stramonium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
NADPH | - |
Datura stramonium |  |
| 0.06 | - |
tropinone | pH 7.5 | Datura stramonium | |
| 0.063 | - |
tropinone | pH 7.9 | Datura stramonium | |
| 0.07 | - |
tropinone | pH 6.7 | Datura stramonium | |
| 0.1 | - |
tropinone | pH 7.0 | Datura stramonium | |
| 0.11 | - |
tropinone | pH 6.4 | Datura stramonium | |
| 0.33 | - |
tropinone | pH 5.95 | Datura stramonium | |
| 1.25 | - |
tropinone | pH 5.4 | Datura stramonium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
3 * 27500 | Atropa belladonna |
| 27700 | - |
3 * 27700, SDS-PAGE | Datura stramonium |
| 29000 | - |
3 * 29000 | Datura stramonium |
| 77700 | - |
gel filtration | Datura stramonium |
| 78500 | - |
- |
Atropa belladonna |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | Atropa belladonna | - |
pseudotropine + NADP+ | - |
? | |
| tropinone + NADPH + H+ | Datura stramonium | pseudotropine oxidation never observed | pseudotropine + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Atropa belladonna | - |
- |
- |
| Datura stramonium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Datura stramonium |
- |
Atropa belladonna |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Atropa belladonna | - |
| root | hairy roots | Datura stramonium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.16 | - |
- |
Datura stramonium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-ethyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
| 6-hydroxytropinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| N-methyl-4-piperidone + NADPH | - |
Datura stramonium | N-methylpiperidin-4-ol + NADP+ | - |
? | |
| N-propyl-4-piperidone + NADPH | - |
Datura stramonium | N-propylpiperidin-4-ol + NADP+ | - |
? | |
| tetrahydrothiopyran-4-one + NADPH | - |
Datura stramonium | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
| tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
? | |
| tropinone + NADPH + H+ | pseudotropine oxidation never observed | Datura stramonium | pseudotropine + NADP+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 27500 | Atropa belladonna |
| trimer | 3 * 29000 | Datura stramonium |
| trimer | 3 * 27700, SDS-PAGE | Datura stramonium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | optimum for tetrahydrothiopyran-4-one reduction | Datura stramonium |
| 6.25 | - |
- |
Datura stramonium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | keeps 90% of maximal activity in the range pH 5.2-8.2 | Datura stramonium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Datura stramonium | |
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