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Literature summary for 1.1.1.233 extracted from

  • Horiuchi, T.; Kurokawa, T.
    Purification and properties of N-acyl-D-mannosamine dehydrogenase from Flavobacterium sp. 141-8 (1988), J. Biochem., 104, 466-471.
    View publication on PubMed

Application

Application Comment Organism
analysis quantitative determination of N-acetylneuraminic acid Flavobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed under control of a lac-promoter in Escherichia coli JM109, the Escherichia coli transformants JM109(pNAM307) and JM109(pNAM308) show up to 200-fold higher activity than Flavobacterium sp. Flavobacterium sp.

General Stability

General Stability Organism
inactivated by frequent freezing and thawing Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
AMP slight inhibition Flavobacterium sp.
Cd2+ slight inhibition Flavobacterium sp.
Cu2+ slight inhibition Flavobacterium sp.
Hg2+ strong inhibitor Flavobacterium sp.
Mn2+ slight inhibition Flavobacterium sp.
additional information no inhibition with metal-chelating or SH-group-blocking reagents, e.g. EDTA, 2,2'-bipyridyl, 8-hydroxyquinoline, PCMB Flavobacterium sp.
Ni2+ slight inhibition Flavobacterium sp.
SDS strong inhibitor Flavobacterium sp.
Zn2+ slight inhibition Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
NAD+
-
Flavobacterium sp.
1
-
N-acetyl-D-mannosamine
-
Flavobacterium sp.
13.3
-
N-glycolyl-D-mannosamine
-
Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27470
-
calculated from amino acid sequence Flavobacterium sp.
29000
-
SDS-PAGE Flavobacterium sp.
120000
-
gel filtration Flavobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine + NAD+ Flavobacterium sp. reaction in N-acetyl-D-mannosamine catabolism N-acetyl-D-mannosaminolactone + NADH + H+
-
?
N-acetyl-D-mannosamine + NAD+ Flavobacterium sp. 141-8 reaction in N-acetyl-D-mannosamine catabolism N-acetyl-D-mannosaminolactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
141-8
-
Flavobacterium sp. 141-8
-
141-8
-

Purification (Commentary)

Purification (Comment) Organism
-
Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
crude extract Flavobacterium sp.
340
-
purified enzyme Flavobacterium sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least several months Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine + NAD+
-
Flavobacterium sp. N-acetyl-D-mannosaminolactone + NADH + H+
-
ir
N-acetyl-D-mannosamine + NAD+ reaction in N-acetyl-D-mannosamine catabolism Flavobacterium sp. N-acetyl-D-mannosaminolactone + NADH + H+
-
?
N-acetyl-D-mannosamine + NAD+
-
Flavobacterium sp. 141-8 N-acetyl-D-mannosaminolactone + NADH + H+
-
ir
N-acetyl-D-mannosamine + NAD+ reaction in N-acetyl-D-mannosamine catabolism Flavobacterium sp. 141-8 N-acetyl-D-mannosaminolactone + NADH + H+
-
?
N-glycolyl-D-mannosamine + NAD+ oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation Flavobacterium sp. N-glycolyl-D-mannosaminolactone + NADH
-
?
N-glycolyl-D-mannosamine + NAD+ oxidation at 62% the rate of N-acetyl-D-mannosamine oxidation Flavobacterium sp. 141-8 N-glycolyl-D-mannosaminolactone + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 29000 Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
inactivation at pH 5.0 after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h Flavobacterium sp.
42
-
and below, stable for at least 10 min Flavobacterium sp.
50
-
t1/2: 10 min Flavobacterium sp.
60
-
complete inactivation after 10 min Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 11.5
-
Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
inactivation at 25°C after 16 h, gradually reversed to about 80% of original activity by shifting the pH back to 8.2 and keeping it at room temperature for 36 h Flavobacterium sp.
7
-
and below, more than 70% loss of activity Flavobacterium sp.
8.5 9.5 more stable than at pH 8.2 at 45°C, 10 min Flavobacterium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+, ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate Flavobacterium sp.
NAD+ specific for Flavobacterium sp.