Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.23 extracted from

  • Nagai, A.; Ohta, D.
    Histidinol dehydrogenase loses its catalytic function through the mutation of His261-Asn due to its inability to ligate the essential Zn (1994), J. Biochem., 115, 22-25.
    View publication on PubMed
Search for more literature for 1.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda, SF9 cells Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
H261N mutation causes complete loss of Zn and activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 L-histidinal
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.0037
-
 L-histidinal H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium
0.0072
-
 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.24
-
 NAD+ H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential for activity, one Zn atom per subunit Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidinal + NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium reaction intermediate L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinal + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + NADH + H+
-
 ?
L-histidinal + NAD+ + H2O reaction intermediate Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + NADH + H+
-
 ?
L-histidinol + 2 NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 L-histidinal H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
 L-histidinal
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium