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Literature summary for 1.1.1.227 extracted from

  • Khine, A.A.; Lu, P.C.; Ko, T.P.; Huang, K.F.; Chen, H.P.
    Cloning, expression, identification and characterization of borneol dehydrogenase isozymes in Pseudomonas sp. TCU-HL1 (2020), Protein Expr. Purif., 175, 105715 .
    View publication on PubMed
Search for more literature for 1.1.1.227

Application

Application Comment Organism
synthesis production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium Pseudomonas sp. TCU-HL1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp. TCU-HL1

Inhibitors

Inhibitors Comment Organism Structure
(-)-Camphor
-
 Pseudomonas sp. TCU-HL1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 (-)-borneol soluble recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1
1.5
-
 (-)-borneol refolded recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. TCU-HL1 A0A1B3EB36 isoform BDH1, cf. EC 1.1.1.198
-

Purification (Commentary)

Purification (Comment) Organism
production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium Pseudomonas sp. TCU-HL1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-borneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (-)-camphor + NADH + H+
-
 ?
additional information (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 Pseudomonas sp. TCU-HL1 ?
-
-

Synonyms

Synonyms Comment Organism
BDH1 cf. EC 1.1.1.198 Pseudomonas sp. TCU-HL1
THL1_4180
-
 Pseudomonas sp. TCU-HL1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 (-)-borneol refolded recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1
12.4
-
 (-)-borneol soluble recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
 (-)-Camphor soluble recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1
0.52
-
 (-)-Camphor refolded recombinant protein, pH 7, 25°C Pseudomonas sp. TCU-HL1